ArticlePlant physiology2011
The folylpolyglutamate synthetase plastidial isoform is required for postembryonic root development in Arabidopsis.
Article in Plant physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 66 citations in OpenAlex.
- Folate depletion impact on the cell cycle results in restricted primary root growth in Arabidopsis.Plant molecular biology · 2025Article
- Arabidopsis Root Development Regulation by the Endogenous Folate Precursor, Para-Aminobenzoic Acid, via Modulation of the Root Cell Cycle.Plants (Basel, Switzerland) · 2023Article
- Metabolic network changes during skotomorphogenesis inPlant direct · 2022Article
- Folic Acid Reinforces Maize Tolerance to Sodic-Alkaline Stress through Modulation of Growth, Biochemical and Molecular Mechanisms.Life (Basel, Switzerland) · 2022Article
- Folic Acid Confers Tolerance against Salt Stress-Induced Oxidative Damages in Snap Beans through Regulation Growth, Metabolites, Antioxidant Machinery and Gene Expression.Plants (Basel, Switzerland) · 2022Article
- Polymer-coated carbon nanotube hybrids with functional peptides for gene delivery into plant mitochondria.Nature communications · 2022Article
- A starting guide to root ecology: strengthening ecological concepts and standardising root classification, sampling, processing and trait measurements.The New phytologist · 2021Review
- A 1232 bp upstream sequence of glutamine synthetase 1b from Eichhornia crassipes is a root-preferential promoter sequence.BMC plant biology · 2021Article
- Interkingdom Comparison of Threonine Metabolism for Stem Cell Maintenance in Plants and Animals.Frontiers in cell and developmental biology · 2021Review
- Interactions between metabolism and chromatin in plant models.Molecular metabolism · 2020Review
- Elimination of human folypolyglutamate synthetase alters programming and plasticity of somatic cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Simultaneous extraction and determination of mono-/polyglutamyl folates using high-performance liquid chromatography-tandem mass spectrometry and its applications in starchy crops.Analytical and bioanalytical chemistry · 2019Article
- Combining loss of function ofBiotechnology for biofuels · 2019Article
- Dihydrofolate Reductase/Thymidylate Synthase Fine-Tunes the Folate Status and Controls Redox Homeostasis in Plants.The Plant cell · 2017Article
- The MEDIATOR genes MED12 and MED13 control Arabidopsis root system configuration influencing sugar and auxin responses.Plant molecular biology · 2017Article
- Plastidial Folate Prevents Starch Biosynthesis Triggered by Sugar Influx into Non-Photosynthetic Plastids of Arabidopsis.Plant & cell physiology · 2017Article
- Tetrahydrofolate Modulates Floral Transition through Epigenetic Silencing.Plant physiology · 2017Article
- Folates in Plants: Research Advances and Progress in Crop Biofortification.Frontiers in chemistry · 2017Review
- MTHFD1 controls DNA methylation in Arabidopsis.Nature communications · 2016Article
- Identification of Polymorphisms Associated with Drought Adaptation QTL in Brassica napus by Resequencing.G3 (Bethesda, Md.) · 2016Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A recessive Arabidopsis (Arabidopsis thaliana) mutant with short primary roots and root hairs was identified from a forward genetic screen. The disrupted gene in the mutant encoded the plastidial isoform of folylpolyglutamate synthetase (FPGS), previously designated as AtDFB, an enzyme that catalyzes the addition of glutamate residues to the folate molecule to form folylpolyglutamates. The short primary root of atdfb was associated with a disorganized quiescent center, dissipated auxin gradient in the root cap, bundled actin cytoskeleton, and reduced cell division and expansion. The accumulation of monoglutamylated forms of some folate classes in atdfb was consistent with impaired FPGS function. The observed cellular defects in roots of atdfb underscore the essential role of folylpolyglutamates in the highly compartmentalized one-carbon transfer reactions (C1 metabolism) that lead to the biosynthesis of compounds required for metabolically active cells found in the growing root apex. Indeed, metabolic profiling uncovered a depletion of several amino acids and nucleotides in atdfb indicative of broad alterations in metabolism. Methionine and purines, which are synthesized de novo in plastids via C1 enzymatic reactions, were particularly depleted. The root growth and quiescent center defects of atdfb were rescued by exogenous application of 5-formyl-tetrahydrofolate, a stable folate that was readily converted to metabolically active folates. Collectively, our results indicate that AtDFB is the predominant FPGS isoform that generates polyglutamylated folate cofactors to support C1 metabolism required for meristem maintenance and cell expansion during postembryonic root development in Arabidopsis.
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