ArticleBMC microbiology2011
Lack of evidence for a role of hydrophobins in conferring surface hydrophobicity to conidia and hyphae of Botrytis cinerea.
Article in BMC microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 47 citations in OpenAlex.
- Fungal Hydrophobins: Taxonomic Distribution, Functional Roles, Physicochemical Properties, and Biotechnological Applications.Journal of fungi (Basel, Switzerland) · 2026Review
- Reverse Genetic Analyses of Hydrophobins inPathogens (Basel, Switzerland) · 2025Article
- Hydrophobins inFrontiers in fungal biology · 2025Article
- TheJournal of fungi (Basel, Switzerland) · 2022Article
- Isolation and Characterization of a Novel Hydrophobin, Sa-HFB1, with Antifungal Activity from an Alkaliphilic Fungus,Journal of fungi (Basel, Switzerland) · 2022Article
- Self-Cleaning Biomimetic Surfaces-The Effect of Microstructure and Hydrophobicity on Conidia Repellence.Materials (Basel, Switzerland) · 2022Article
- The G protein subunit α1, CaGα1, mediates ethylene sensing of mango anthracnose pathogenFrontiers in microbiology · 2022Article
- Tracking of Diversity and Evolution in the Brown Rot FungiFrontiers in microbiology · 2022Article
- Hydrophobin CmHYD1 Is Involved in Conidiation, Infection and Primordium Formation, and Regulated by GATA Transcription Factor CmAreA in Edible Fungus,Journal of fungi (Basel, Switzerland) · 2021Article
- The Destructive Fungal PathogenPathogens (Basel, Switzerland) · 2020Review
- Characterization of Growth Morphology and Pathology, and Draft Genome Sequencing ofFrontiers in microbiology · 2020Article
- Different Hydrophobins ofFrontiers in microbiology · 2019Article
- The mycoparasitic fungusEvolutionary applications · 2018Article
- Article
- Evaluation of Pathogenicity of the FungiIndian journal of microbiology · 2016Article
- Impact of photocatalysis on fungal cells: depiction of cellular and molecular effects on Saccharomyces cerevisiae.Applied and environmental microbiology · 2014Article
- Hydrophobins are required for conidial hydrophobicity and plant root colonization in the fungal biocontrol agent Clonostachys rosea.BMC microbiology · 2014Article
- Hydrophobin genes of the entomopathogenic fungus, Metarhizium brunneum, are differentially expressed and corresponding mutants are decreased in virulence.Current genetics · 2012Article
- Development of an indirect competitive enzyme-linked immunosorbent assay applied to the Botrytis cinerea quantification in tissues of postharvest fruits.BMC microbiology · 2011Article
- Diffusion of hydrophobin proteins in solution and interactions with a graphite surface.BMC biophysics · 2011Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHydrophobins are small, cysteine rich, surface active proteins secreted by filamentous fungi, forming hydrophobic layers on the walls of aerial mycelia and spores. Hydrophobin mutants in a variety of fungi have been described to show 'easily wettable' phenotypes, indicating that hydrophobins play a general role in conferring surface hydrophobicity to aerial hyphae and spores.
resultsIn the genome of the grey mould fungus Botrytis cinerea, genes encoding three hydrophobins and six hydrophobin-like proteins were identified. Expression analyses revealed low or no expression of these genes in conidia, while some of them showed increased or specific expression in other stages, such as sclerotia or fruiting bodies. Bhp1 belongs to the class I hydrophobins, whereas Bhp2 and Bhp3 are members of hydrophobin class II. Single, double and triple hydrophobin knock-out mutants were constructed by consecutively deleting bhp1, bhp2 and bhp3. In addition, a mutant in the hydrophobin-like gene bhl1 was generated. The mutants were tested for germination and growth under different conditions, formation of sclerotia, ability to penetrate and infect host tissue, and for spore and mycelium surface properties. Surprisingly, none of the B. cinerea hydrophobin mutants showed obvious phenotypic defects in any of these characters. Scanning electron microscopy of the hydrophobic conidial surfaces did not reveal evidence for the presence of typical hydrophobin 'rodlet' layers.
conclusionsThese data provide evidence that in B. cinerea, hydrophobins are not involved in conferring surface hydrophobicity to conidia and aerial hyphae, and challenge their universal role in filamentous fungi. The function of some of these proteins in sclerotia and fruiting bodies remains to be investigated.
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