ArticleFEMS immunology and medical microbiology2011
Mutational and transcriptional analyses of the Staphylococcus aureus low-affinity proline transporter OpuD during in vitro growth and infection of murine tissues.
Article in FEMS immunology and medical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Essential role of proline synthesis and the one-carbon metabolism pathways for systemic virulence ofmBio · 2024Article
- Proline transporters ProT and PutP are required for Staphylococcus aureus infection.PLoS pathogens · 2023Article
- Genomic insight into strategy, interaction and evolution of nitrifiers in metabolizing key labile-dissolved organic nitrogen in different environmental niches.Frontiers in microbiology · 2023Article
- Proline Transport and Growth Changes in Proline Transport Mutants ofMicroorganisms · 2022Article
- Exploring amino acid and peptide transporters as therapeutic targets to attenuate virulence and antibiotic resistance in Staphylococcus aureus.PLoS pathogens · 2021Article
- Genome-Based Classification of Strain 16-SW-7, a Marine Bacterium Capable of Converting B Red Blood Cells, asFrontiers in microbiology · 2021Article
- High-throughput transposon sequencing highlights the cell wall as an important barrier for osmotic stress in methicillin resistant Staphylococcus aureus and underlines a tailored response to different osmotic stressors.Molecular microbiology · 2020Article
- Proteomic and Metabolomic Analyses of a Tea-Tree Oil-SelectedAntibiotics (Basel, Switzerland) · 2019Article
- Role of Proline in Pathogen and Host Interactions.Antioxidants & redox signaling · 2019Review
- Cyclic di-adenosine monophosphate (c-di-AMP) is required for osmotic regulation inThe Journal of biological chemistry · 2018Article
- Comparative virulence studies and transcriptome analysis of Staphylococcus aureus strains isolated from animals.Scientific reports · 2016Article
- The second messenger c-di-AMP inhibits the osmolyte uptake system OpuC in Staphylococcus aureus.Science signaling · 2016Article
- Alternating-access mechanism in conformationally asymmetric trimers of the betaine transporter BetP.Nature · 2012Article
- Osmolyte transport inWorld journal of clinical infectious diseasesArticle
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Staphylococcus aureus continues to be a major health problem. This species' requirement for proline and proline transport from the extracellular environment is not well understood. Here, we identify a S. aureus low-affinity proline transport gene (opuD) with homology to the OpuD protein of Bacillus subtilis. Mutation of the opuD gene caused a significant decline in proline uptake under low-affinity conditions as compared with wild type, but the opuD mutant strain showed no significant attenuation in a murine abscess model of infection. The S. aureus opuD gene was transcriptionally activated during growth in moderate osmolarity media with high levels of proline or glycine betaine independent of SigB. In murine abscesses, the opuD gene was activated at a later time point, whereas the opuD expression dropped over the course of an 18-h period within murine urinary tracts. Transcriptional regulation of opuD in S. aureus appears to be coordinated within this species when grown in moderate to high NaCl environments, but the level of extracellular proline had a marked effect on expression of this proline transport gene. The differential regulation of proline transport genes in S. aureus may be an adaptation for life in a variety of environments, including survival within the human body.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.