ArticleStem cells and development2011
Transduction of Wnt11 promotes mesenchymal stem cell transdifferentiation into cardiac phenotypes.
Article in Stem cells and development, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- Cardiac gene delivery using ultrasound: State of the field.Molecular therapy. Methods & clinical development · 2024Review
- Noncanonical Wnt Signaling Promotes Myofibroblast Differentiation in Pulmonary Fibrosis.American journal of respiratory cell and molecular biology · 2021Article
- The role of Sfrp and DKK proteins in cardiomyocyte development.Physiological reports · 2021Review
- Mapping current research and identifying hotspots on mesenchymal stem cells in cardiovascular disease.Stem cell research & therapy · 2020Article
- In vitro differentiation of W8B2Cellular & molecular biology letters · 2020Article
- Article
- Yixin-Shu facilitated cardiac-like differentiation of mesenchymal stem cellsRSC advances · 2018Article
- WNT Signaling in Cardiac and Vascular Disease.Pharmacological reviews · 2018Review
- Wnt11 regulates cardiac chamber development and disease during perinatal maturation.JCI insight · 2017Article
- Stem Cell-Derived Exosomes, Autophagy, Extracellular Matrix Turnover, and miRNAs in Cardiac Regeneration during Stem Cell Therapy.Stem cell reviews and reports · 2017Review
- Cardiac gene therapy: are we there yet?Gene therapy · 2016Review
- Rapid fusion between mesenchymal stem cells and cardiomyocytes yields electrically active, non-contractile hybrid cells.Scientific reports · 2015Article
- Overexpression of Wnt11 promotes chondrogenic differentiation of bone marrow-derived mesenchymal stem cells in synergism with TGF-β.Molecular and cellular biochemistry · 2014Article
- A cocktail method for promoting cardiomyocyte differentiation from bone marrow-derived mesenchymal stem cells.Stem cells international · 2014Article
- Article
- Oxidative stress-induced Notch1 signaling promotes cardiogenic gene expression in mesenchymal stem cells.Stem cell research & therapy · 2013Article
- What's new in regenerative medicine: split up of the mesenchymal stem cell family promises new hope for cardiovascular repair.Journal of cardiovascular translational research · 2012Review
- Conversion of human bone marrow-derived mesenchymal stem cells into tendon progenitor cells by ectopic expression of scleraxis.Stem cells and development · 2012Article
- Paracrine effect of Wnt11-overexpressing mesenchymal stem cells on ischemic injury.Stem cells and development · 2012Article
- GATA-4 promotes myocardial transdifferentiation of mesenchymal stromal cells via up-regulating IGFBP-4.Cytotherapy · 2011Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Transplantation of mesenchymal stem cells (MSCs) has emerged as a potential treatment for ischemic heart repair. Previous studies have suggested that Wnt11 plays a critical role in cardiac specification and morphogenesis. In this study, we examined whether transduction of Wnt11 directly increases MSC differentiation into cardiac phenotypes. MSCs harvested from rat bone marrow were transduced with both Wnt11 and green fluorescent protein (GFP) (MSC(Wnt11)) using the murine stem cell virus (pMSCV) retroviral expression system; control cells were only GFP-transfected (MSC(Null)). Compared with control cells, MSC(Wnt11) was shown to have higher expression of Wnt11 by immunofluorescence, real-time polymerase chain reaction, and western blotting. MSC(Wnt11) shows a higher expression of cardiac-specific genes, including GATA-4, brain natriuretic peptide (BNP), islet-1, and α-actinin, after being cultured with cardiomyocytes (CMs) isolated from ventricles of neonatal (1-3 day) SD rats. Some MSC(Wnt11) were positive for α-actinin when MSCs were cocultured with native CMs for 7 days. Electron microscopy further confirmed the appearance of sarcomeres in MSC(Wnt11). Connexin 43 was found between GFP-positive MSCs and neonatal rat CMs labeled with red fluorescent probe PKH26. The transdifferentiation rate was significantly higher in MSC(Wnt11) than in MSC(Null), as assessed by flow cytometry. Functional studies indicated that the differentiation of MSC(Wnt11) was diminished by knockdown of GATA-4 with GATA-4-siRNA. Transduction of Wnt11 into MSCs increases their differentiation into CMs by upregulating GATA-4.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.