ArticleMolecules (Basel, Switzerland)2011
White spot syndrome virus Orf514 encodes a bona fide DNA polymerase.
Article in Molecules (Basel, Switzerland), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 8 citations in OpenAlex.
- A review on molecular detection techniques of white spot syndrome virus: Perspectives of problems and solutions in shrimp farming.Veterinary medicine and science · 2023Review
- Molecular Mechanisms of White Spot Syndrome Virus Infection and Perspectives on Treatments.Viruses · 2016Review
- A novel viral thymidylate kinase with dual kinase activity.Journal of bioenergetics and biomembranes · 2015Article
- Crystal structure of the shrimp proliferating cell nuclear antigen: structural complementarity with WSSV DNA polymerase PIP-box.PloS one · 2014Article
- Molecular modeling and expression of the Litopenaeus vannamei proliferating cell nuclear antigen (PCNA) after white spot syndrome virus shrimp infection.Results in immunology · 2011Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
White spot syndrome virus (WSSV) is the causative agent of white spot syndrome, one of the most devastating diseases in shrimp aquaculture. The genome of WSSV includes a gene that encodes a putative family B DNA polymerase (ORF514), which is 16% identical in amino acid sequence to the Herpes virus 1 DNA polymerase. The aim of this work was to demonstrate the activity of the WSSV ORF514-encoded protein as a DNA polymerase and hence a putative antiviral target. A 3.5 kbp fragment encoding the conserved polymerase and exonuclease domains of ORF514 was overexpressed in bacteria. The recombinant protein showed polymerase activity but with very low level of processivity. Molecular modeling of the catalytic protein core encoded in ORF514 revealed a canonical polymerase fold. Amino acid sequence alignments of ORF514 indicate the presence of a putative PIP box, suggesting that the encoded putative DNA polymerase may use a host processivity factor for optimal activity. We postulate that WSSV ORF514 encodes a bona fide DNA polymerase that requires accessory proteins for activity and maybe target for drugs or compounds that inhibit viral DNA replication.
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