ArticleNucleic acids research2011
An upstream ORF with non-AUG start codon is translated in vivo but dispensable for translational control of GCN4 mRNA.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
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- Control of translation by eukaryotic mRNA transcript leaders-Insights from high-throughput assays and computational modeling.Wiley interdisciplinary reviews. RNA · 2021Review
- Methylated PP2A stabilizes Gcn4 to enable a methionine-induced anabolic program.The Journal of biological chemistry · 2020Article
- Selective Translation Complex Profiling Reveals Staged Initiation and Co-translational Assembly of Initiation Factor Complexes.Molecular cell · 2020Article
- Evidence for a novel overlapping coding sequence in POLG initiated at a CUG start codon.BMC genetics · 2020Article
- Temperature-dependent regulation of upstream open reading frame translation in S. cerevisiae.BMC biology · 2019Article
- Control of Translation at the Initiation Phase During Glucose Starvation in Yeast.International journal of molecular sciences · 2019Review
- Non-AUG translation: a new start for protein synthesis in eukaryotes.Genes & development · 2017Review
- Translational control by 5'-untranslated regions of eukaryotic mRNAs.Science (New York, N.Y.) · 2016Review
- Review
- Transcriptional regulation analysis and the potential transcription regulator site in the extended KAP6.1 promoter in sheep.Molecular biology reports · 2014Article
- An upstream open reading frame regulates LST1 expression during monocyte differentiation.PloS one · 2014Article
- KSHV 2.0: a comprehensive annotation of the Kaposi's sarcoma-associated herpesvirus genome using next-generation sequencing reveals novel genomic and functional features.PLoS pathogens · 2014Article
- Deep proteome coverage based on ribosome profiling aids mass spectrometry-based protein and peptide discovery and provides evidence of alternative translation products and near-cognate translation initiation events.Molecular & cellular proteomics : MCP · 2013Article
- The stringency of start codon selection in the filamentous fungus Neurospora crassa.The Journal of biological chemistry · 2013Article
- Peptidomic discovery of short open reading frame-encoded peptides in human cells.Nature chemical biology · 2013Article
- The expression of a naturally occurring, truncated allele of an α-SNAP gene suppresses plant parasitic nematode infection.Plant molecular biology · 2012Article
- New insights into translational regulation in the endoplasmic reticulum unfolded protein response.Cold Spring Harbor perspectives in biology · 2012Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Genome-wide analysis of ribosome locations in mRNAs of Saccharomyces cerevisiae has revealed the translation of upstream open reading frames that initiate with near-cognate start codons in many transcripts. Two such non-translation initiation codon (AUG)-initiated upstream open reading frames (uORFs) (nAuORFs 1 and 2) occur in GCN4 mRNA upstream of the four AUG-initiated uORFs (uORFs 1-4) that regulate GCN4 translation. We verified that nAuORF2 is translated in vivo by demonstrating β-galactosidase production from lacZ coding sequences fused to nAuORF2, in a manner abolished by replacing its non-AUG initiation codon (AUA) start codon with the non-cognate triplet AAA, whereas translation of nAuORF1 was not detected. Importantly, replacing the near-cognate start codons of both nAuORFs with non-cognate triplets had little or no effect on the repression of GCN4 translation in non-starved cells, nor on its derepression in response to histidine limitation, nutritional shift-down or treatment with rapamycin, hydrogen peroxide or methyl methanesulfonate. Additionally, we found no evidence that initiation from the AUA codon of nAuORF2 is substantially elevated, or dependent on Gcn2, the sole eIF2α kinase of yeast, in histidine-deprived cells. Thus, although nAuORF2 is translated in vivo, it appears that this event is not stimulated by eIF2α phosphorylation nor significantly influences GCN4 translational induction under various starvation or stress conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.