ArticleNucleic acids research2011
Molecular determinants of origin discrimination by Orc1 initiators in archaea.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 42 citations in OpenAlex.
- Dissection of Functional Domains of Orc1-2, the Archaeal Global DNA Damage-Responsive Regulator.International journal of molecular sciences · 2022Article
- G-Quadruplexes in the Archaea Domain.Biomolecules · 2020Article
- Origins of DNA replication.PLoS genetics · 2019Review
- From structure to mechanism-understanding initiation of DNA replication.Genes & development · 2017Review
- Mechanisms and regulation of DNA replication initiation in eukaryotes.Critical reviews in biochemistry and molecular biology · 2017Review
- Diversity of DNA Replication in the Archaea.Genes · 2017Review
- Genome Replication inFrontiers in microbiology · 2017Article
- Diverged composition and regulation of the Trypanosoma brucei origin recognition complex that mediates DNA replication initiation.Nucleic acids research · 2016Article
- Transposons to toxins: the provenance, architecture and diversification of a widespread class of eukaryotic effectors.Nucleic acids research · 2016Article
- DNA sequence templates adjacent nucleosome and ORC sites at gene amplification origins in Drosophila.Nucleic acids research · 2015Article
- Specific binding of eukaryotic ORC to DNA replication origins depends on highly conserved basic residues.Scientific reports · 2015Article
- Article
- Multiple replication origins with diverse control mechanisms in Haloarcula hispanica.Nucleic acids research · 2014Article
- Archaeal chromosome biology.Journal of molecular microbiology and biotechnology · 2014Review
- DNA replication origins in archaea.Frontiers in microbiology · 2014Review
- Characterization of the replication initiator Orc1/Cdc6 from the Archaeon Picrophilus torridus.Journal of bacteriology · 2014Article
- DNA replication origins.Cold Spring Harbor perspectives in biology · 2013Review
- The cell cycle of archaea.Nature reviews. Microbiology · 2013Review
- Genomics and genetics of Sulfolobus islandicus LAL14/1, a model hyperthermophilic archaeon.Open biology · 2013Article
- Specificity and function of archaeal DNA replication initiator proteins.Cell reports · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Unlike bacteria, many eukaryotes initiate DNA replication from genomic sites that lack apparent sequence conservation. These loci are identified and bound by the origin recognition complex (ORC), and subsequently activated by a cascade of events that includes recruitment of an additional factor, Cdc6. Archaeal organisms generally possess one or more Orc1/Cdc6 homologs, belonging to the Initiator clade of ATPases associated with various cellular activities (AAA(+)) superfamily; however, these proteins recognize specific sequences within replication origins. Atomic resolution studies have shown that archaeal Orc1 proteins contact double-stranded DNA through an N-terminal AAA(+) domain and a C-terminal winged-helix domain (WHD), but use remarkably few base-specific contacts. To investigate the biochemical effects of these associations, we mutated the DNA-interacting elements of the Orc1-1 and Orc1-3 paralogs from the archaeon Sulfolobus solfataricus, and tested their effect on origin binding and deformation. We find that the AAA(+) domain has an unpredicted role in controlling the sequence selectivity of DNA binding, despite an absence of base-specific contacts to this region. Our results show that both the WHD and ATPase region influence origin recognition by Orc1/Cdc6, and suggest that not only DNA sequence, but also local DNA structure help define archaeal initiator binding sites.
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