ArticleThe Journal of molecular diagnostics : JMD2011
Multiplexed methylation profiles of tumor suppressor genes in bladder cancer.
Article in The Journal of molecular diagnostics : JMD, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 2 of them syntheses that pooled it.
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Who cites it
22 citing papers in PubMed, 2 syntheses or guidelines pooled it, 48 citations in OpenAlex.
- Intravesical gemcitabine versus mitomycin for non-muscle invasive bladder cancer: a systematic review and meta-analysis of randomized controlled trial.BMC urology · 2020Pooled it
- Clinical significance of CDH13 promoter methylation as a biomarker for bladder cancer: a meta-analysis.BMC urology · 2016Pooled it
- Methylation and Expression Analysis of POU4F2, HOXA9, RBM46, and TSGA10 Genes in Bladder Cancer Using Methyl-Sensitive Restriction Enzyme PCR (MSRE-PCR).Cancer management and research · 2026Article
- Diagnostic Values of Electrochemiluminescent Detection of Urinary CYFRA21-1 and FDP and Their Combined Detection in Bladder Cancer.Cancer medicine · 2025Article
- Significance of Endocan Expression in Various Types of Epithelial Ovarian Tumors.Iranian journal of pathology · 2022Article
- Diagnostic and Therapeutic Implications of Sortilin Expressed on the Surface of Bladder Carcinoma Cells.Iranian journal of pathology · 2022Article
- Epigenome-Wide DNA Methylation Profiling Identifies Differential Methylation Biomarkers in High-Grade Bladder Cancer.Translational oncology · 2017Article
- Analysis of bladder cancer tumor CpG methylation and gene expression within The Cancer Genome Atlas identifiesAmerican journal of cancer research · 2017Article
- DNA Methylation and Urological Cancer, a Step Towards Personalized Medicine: Current and Future Prospects.Molecular diagnosis & therapy · 2016Review
- Methylation pattern analysis in prostate cancer tissue: identification of biomarkers using an MS-MLPA approach.Journal of translational medicine · 2016Article
- Can we use methylation markers as diagnostic and prognostic indicators for bladder cancer?Investigative and clinical urology · 2016Review
- Methylation of tumor suppressor genes in a novel panel predicts clinical outcome in paraffin-embedded bladder tumors.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2014Article
- DNA Methylation profiles as predictors of recurrence in non muscle invasive bladder cancer: an MS-MLPA approach.Journal of experimental & clinical cancer research : CR · 2013Article
- Molecular signatures in urologic tumors.International journal of molecular sciences · 2013Review
- DNA methylation-based biomarkers in bladder cancer.Nature reviews. Urology · 2013Review
- DNA methyltransferases, DNA damage repair, and cancer.Advances in experimental medicine and biology · 2013Review
- Cross-contamination of a UROtsa stock with T24 cells--molecular comparison of different cell lines and stocks.PloS one · 2013Article
- Diagnostic utility of MS-MLPA in DNA methylation profiling of adenocarcinomas and neuroendocrine carcinomas of the colon-rectum.Virchows Archiv : an international journal of pathology · 2013Article
- Hypermethylation in bladder cancer: biological pathways and translational applications.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2012Article
- Emerging critical role of molecular testing in diagnostic genitourinary pathology.Archives of pathology & laboratory medicine · 2012Review
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Changes in DNA methylation of tumor suppressors can occur early in carcinogenesis and are potentially important early indicators of cancer. The objective of this study was to assess the methylation of 25 tumor suppressor genes in bladder cancer using a methylation-specific (MS) multiplex ligation-dependent probe amplification assay (MLPA). Initial analyses in bladder cancer cell lines (n = 14) and fresh-frozen primary bladder tumor specimens (n = 31) supported the panel of genes selected being altered in bladder cancer. The process of MS-MLPA was optimized for its application in body fluids using two independent training and validation sets of urinary specimens (n = 146), including patients with bladder cancer (n = 96) and controls (n = 50). BRCA1 (71.0%), WT1 (38.7%), and RARB (38.7%) were the most frequently methylated genes in bladder tumors, with WT1 methylation being significantly associated with tumor stage (P = 0.011). WT1 and PAX5A were identified as methylated tumor suppressors. In addition, BRCA1, WT1, and RARB were the most frequently methylated genes in urinary specimens. Receiver operating characteristic curve analyses revealed significant diagnostic accuracies in both urinary sets for BRCA1, RARB, and WT1. The novelty of this report relates to applying MS-MLPA, a multiplexed methylation technique, for tumor suppressors in bladder cancer and body fluids. Methylation profiles of tumor suppressor genes were clinically relevant for histopathological stratification of bladder tumors and offered a noninvasive diagnostic strategy for the clinical management of patients affected with uroepithelial neoplasias.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.