ArticleMycorrhiza2011
Uvitex2B: a rapid and efficient stain for detection of arbuscular mycorrhizal fungi within plant roots.
Article in Mycorrhiza, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Cellular anatomy of arbuscular mycorrhizal fungi.Current biology : CB · 2025Review
- A historical perspective on mycorrhizal mutualism emphasizing arbuscular mycorrhizas and their emerging challenges.Mycorrhiza · 2021Review
- Effects of growth promoting microorganisms on tomato seedlings growing in different media conditions.PloS one · 2021Article
- Shedding Light on Penetration of Cereal Host Stomata by Wheat Stem Rust Using Improved Methodology.Scientific reports · 2019Article
- Chitotetraose activates the fungal-dependent endosymbiotic signaling pathway in actinorhizal plant species.PloS one · 2019Article
- Auxin perception is required for arbuscule development in arbuscular mycorrhizal symbiosis.Plant physiology · 2014Article
- Combining microtomy and confocal laser scanning microscopy for structural analyses of plant-fungus associations.Mycorrhiza · 2014Article
- Simultaneous specific in planta visualization of root-colonizing fungi using fluorescence in situ hybridization (FISH).Mycorrhiza · 2014Article
- Article
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Authors and funding
10 authors.
Funding
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Abstract
The study of arbuscular mycorrhiza often requires the staining of fungal structures using specific dyes. Fluorescent dyes such as acid fuchsin and wheat germ agglutinin conjugates give excellent results, but these compounds are either hazardous or very expensive. Here, we show that a safer and inexpensive dye, Uvitex2B, can be efficiently used to stain intraradical fungal structures formed by the arbuscular mycorrhizal fungus Glomus intraradices in three plant species: carrot, Casuarina equisetifolia, and Medicago truncatula. The intensity and stability of Uvitex2B allow the acquisition of high-quality images using not only confocal laser scanning microscopy but also epifluorescence microscopy coupled with image deconvolution. Furthermore, we demonstrate that Uvitex2B and β-glucuronidase staining are compatible and can thus be used to reveal arbuscular mycorrhizal structures in the context of promoter activation analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.