ArticleMolecular vision2011
Quantitative assessment of DNA damage directly in lens epithelial cells from senile cataract patients.
Article in Molecular vision, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Aging of the human eye lens: Epigenetic landscape and therapeutic targets in age‑related cataracts (Review).International journal of molecular medicine · 2025Review
- Effects of MMP2 and its inhibitor TIMP2 on DNA damage, apoptosis and senescence of human lens epithelial cells induced by oxidative stress.Journal of bioenergetics and biomembranes · 2024Article
- Preventative Effects ofNutrients · 2023Article
- Analysis of cataract-regulated genes using chemical DNA damage induction in a rat ex vivo model.PloS one · 2022Article
- Article
- Role of biometals in activation of immune cum inflammatory response in ovine ageing eye: a potential model for understanding human geriatric eye diseases.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2021Article
- Long non‑coding RNA NONHSAT143692.2 is involved in oxidative DNA damage repair in the lens by regulating the miR‑4728‑5p/OGG1 axis.International journal of molecular medicine · 2020Article
- Synchrotron-based FTIR microspectroscopy of protein aggregation and lipids peroxidation changes in human cataractous lens epithelial cells.Scientific reports · 2020Article
- Ocular Cell Lines and Genotoxicity Assessment.International journal of environmental research and public health · 2020Review
- lncRNA H19 contributes to oxidative damage repair in the early age-related cataract by regulating miR-29a/TDG axis.Journal of cellular and molecular medicine · 2019Article
- Age-related aqueous humor (AH) and lens epithelial cell/capsule protein carbonylation and AH protein concentration in cataract patients who have pseudoexfoliative diseases.Molecular vision · 2018Article
- PARP-1/PAR Activity in Cultured Human Lens Epithelial Cells Exposed to Two Levels of UVB Light.Photochemistry and photobiology · 2018Article
- CpG site methylation in CRYAA promoter affect transcription factor Sp1 binding in human lens epithelial cells.BMC ophthalmology · 2016Article
- Ultraviolet-B induces ERCC6 repression in lens epithelium cells of age-related nuclear cataract through coordinated DNA hypermethylation and histone deacetylation.Clinical epigenetics · 2016Article
- The Role of the Reactive Oxygen Species and Oxidative Stress in the Pathomechanism of the Age-Related Ocular Diseases and Other Pathologies of the Anterior and Posterior Eye Segments in Adults.Oxidative medicine and cellular longevity · 2016Review
- Screening of potential target genes for cataract by analyzing mRNA expression profile of mouse Hsf4-null lens.BMC ophthalmology · 2015Article
- Epithelial cells as alternative human biomatrices for comet assay.Frontiers in genetics · 2014Review
- Correlation between microRNA-34a levels and lens opacity severity in age-related cataracts.Eye (London, England) · 2013Article
- Serum antioxidative enzymes levels and oxidative stress products in age-related cataract patients.Oxidative medicine and cellular longevity · 2013Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeMost of the studies regarding DNA damage in lens epithelial cells (LECs) of cataract patients have been done on lymphocyte or human LECs cultures. Studies of DNA damage directly in LECs of cataract patients are scanty and, to our knowledge, neither photographic evidence nor has a quantitative assessment of DNA damage have been put forward. In our study, we assessed and quantified DNA damage directly in the LECs of senile cataract patients, right after cataract surgery.
methodsLECs were taken from different morphologic types of senile cataract patients after surgery and DNA damage was immediately assessed by comet assay. Quantitative assessment of DNA damage was conducted using CometScore ™ software.
resultsThere were no prominent comets in most of the LECs of the control subjects, but comets were found in cataractous LECs. DNA fragments in the tail of the comet gave smearing (not banded), which was indicative of chemical damage (i.e., not site specific). DNA damage in the LECs of cataract patients was highly significant (p<0.001). DNA damage in cortical cataracts was significant (p<0.01) when compared to that of nuclear or posterior subcapsular cataracts, but the DNA damage between nuclear and posterior subcapsular cataracts was not significant. Furthermore, we found disrupted nuclear membranes in some of the nuclei in LECs of patients, but not in the control subjects.
conclusionsIn senile cataract patients, LECs DNA was randomly damaged and this type of damage was possible by reactive oxygen species (ROS). The damage was found maximally in the cortical type of cataracts. Oxidative DNA damage of the LECs may be one of the etiology of senile cataractogenesis.
Indexed as
Identifiers
21224996PMC3017798W2167104452What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.