ArticleThe Journal of biological chemistry2011
Silencing of the GDP-D-mannose 3,5-epimerase affects the structure and cross-linking of the pectic polysaccharide rhamnogalacturonan II and plant growth in tomato.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 61 citations in OpenAlex.
- Identification of poplar and spruce GDP-mannose pyrophosphorylases involved in mannan and ascorbic acid biosynthesis.Tree physiology · 2026Article
- Tobacco straw biochar enhances pak choi growth and quality: Lead/cadmium immobilization and soil fertility improvement.iScience · 2026Article
- Storming the barricades of rhamnogalacturonan-II synthesis and function.The Plant cell · 2025Review
- Molecular mechanisms affected by boron deficiency in root and shoot meristems of plants.Journal of experimental botany · 2025Review
- Putative rhamnogalacturonan-II glycosyltransferase identified through callus gene editing which bypasses embryo lethality.Plant physiology · 2024Article
- Multi-regulated GDP-l-galactose phosphorylase calls the tune in ascorbate biosynthesis.Journal of experimental botany · 2024Review
- The ascorbate biosynthesis pathway in plants is known, but there is a way to go with understanding control and functions.Journal of experimental botany · 2024Review
- A mutation in CsGME encoding GDP-mannose 3,5-epimerase results in little and wrinkled leaf in cucumber.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Article
- Effect of light on ascorbic acid biosynthesis and bioinformatics analysis of related genes in Chinese chives.PloS one · 2024Article
- Genetic and biochemical strategies for regulation of L-ascorbic acid biosynthesis in plants through the L-galactose pathway.Frontiers in plant science · 2023Review
- Article
- Protocols for isolating and characterizing polysaccharides from plant cell walls: a case study using rhamnogalacturonan-II.Biotechnology for biofuels · 2021Article
- Golgi-localized membrane protein AtTMN1/EMP12 functions in the deposition of rhamnogalacturonan II and I for cell growth in Arabidopsis.Journal of experimental botany · 2021Article
- The role of GDP-l-galactose phosphorylase in the control of ascorbate biosynthesis.Plant physiology · 2021Article
- Three metabolic pathways are responsible for the accumulation and maintenance of high AsA content in kiwifruit (Actinidia eriantha).BMC genomics · 2021Article
- GDP-Mannose 3,5-Epimerase: A View on Structure, Mechanism, and Industrial Potential.Frontiers in molecular biosciences · 2021Review
- Suppression of Arabidopsis GGLT1 affects growth by reducing the L-galactose content and borate cross-linking of rhamnogalacturonan-II.The Plant journal : for cell and molecular biology · 2018Article
- Vitamin C Content in Fruits: Biosynthesis and Regulation.Frontiers in plant science · 2018Review
- Molecular Evolution of GDP-D-Mannose Epimerase (Frontiers in plant science · 2018Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
L-galactose (L-Gal), a monosaccharide involved in L-ascorbate and rhamnogalacturonan II (RG-II) biosynthesis in plants, is produced in the cytosol by a GDP-D-mannose 3,5-epimerase (GME). It has been recently reported that the partial inactivation of GME induced growth defects affecting both cell division and cell expansion (Gilbert, L., Alhagdow, M., Nunes-Nesi, A., Quemener, B., Guillon, F., Bouchet, B., Faurobert, M., Gouble, B., Page, D., Garcia, V., Petit, J., Stevens, R., Causse, M., Fernie, A. R., Lahaye, M., Rothan, C., and Baldet, P. (2009) Plant J. 60, 499-508). In the present study, we show that the silencing of the two GME genes in tomato leaves resulted in approximately a 60% decrease in terminal L-Gal content in the side chain A of RG-II as well as in a lower capacity of RG-II to perform in muro cross-linking. In addition, we show that unlike supplementation with L-Gal or ascorbate, supplementation of GME-silenced lines with boric acid was able to restore both the wild-type growth phenotype of tomato seedlings and an efficient in muro boron-mediated cross-linking of RG-II. Our findings suggest that developmental phenotypes in GME-deficient lines are due to the structural alteration of RG-II and further underline the crucial role of the cross-linking of RG-II in the formation of the pectic network required for normal plant growth and development.
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