ArticleDevelopmental biology2011
Transgenerational analysis of transcriptional silencing in zebrafish.
Article in Developmental biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 103 papers.
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Who cites it
103 citing papers in PubMed, 185 citations in OpenAlex.
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- Development of a novel simplified Q-system with integrated temporal expression control.bioRxiv : the preprint server for biology · 2026Article
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- Library transgenesis in zebrafish through delayed site-specific mosaic integration for in vivo pooled screening of transgenes.bioRxiv : the preprint server for biology · 2026Article
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- TransTag enables simple and efficient transgene mapping in zebrafish via tagmentation.Cell reports methods · 2025Article
- Characterization of Transgenic Lines Labeling Reticulospinal Neurons in Larval Zebrafish.eNeuro · 2025Article
- Transsynaptic labeling and transcriptional control of zebrafish neural circuits.Nature neuroscience · 2025Article
- CRIMP: a CRISPR/Cas9 insertional mutagenesis protocol and toolkit.Nature communications · 2024Article
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- Selective Cell Ablation Using an Improved Prodrug-Converting Nitroreductase.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Spreading of Tau Protein Does Not Depend on Aggregation Propensity.Journal of molecular neuroscience : MN · 2023Article
- Optogenetic manipulation of Gq- and Gi/o-coupled receptor signaling in neurons and heart muscle cells.eLife · 2023Article
- Circadian regulation of developmental synaptogenesis via the hypocretinergic system.Nature communications · 2023Article
- An arginase 2 promoter transgenic line illuminates immune cell polarisation in zebrafish.Disease models & mechanisms · 2023Article
43 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The yeast Gal4/UAS transcriptional activation system is a powerful tool for regulating gene expression in Drosophila and has been increasing in popularity for developmental studies in zebrafish. It is also useful for studying the basis of de novo transcriptional silencing. Fluorescent reporter genes under the control of multiple tandem copies of the upstream activator sequence (UAS) often show evidence of variegated expression and DNA methylation in transgenic zebrafish embryos. To characterize this systematically, we monitored the progression of transcriptional silencing of UAS-regulated transgenes that differ in their integration sites and in the repetitive nature of the UAS. Transgenic larvae were examined in three generations for tissue-specific expression of a green fluorescent protein (GFP) reporter and DNA methylation at the UAS. Single insertions containing four distinct upstream activator sequences were far less susceptible to methylation than insertions containing fourteen copies of the same UAS. In addition, transgenes that integrated in or adjacent to transposon sequence exhibited silencing regardless of the number of UAS sites included in the transgene. Placement of promoter-driven Gal4 upstream of UAS-regulated responder genes in a single bicistronic construct also appeared to accelerate silencing and methylation. The results demonstrate the utility of the zebrafish for efficient tracking of gene silencing mechanisms across several generations, as well as provide useful guidelines for optimal Gal4-regulated gene expression in organisms subject to DNA methylation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.