ArticleFEMS microbiology letters2011
Flavin mononucleotide (FMN)-based fluorescent protein (FbFP) as reporter for gene expression in the anaerobe Bacteroides fragilis.
Article in FEMS microbiology letters, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 57 citations in OpenAlex.
- Rational Design of a Circularly Permuted Flavin-Based Fluorescent Protein.Chembiochem : a European journal of chemical biology · 2024Article
- Fluorescent Protein-Based Sensors for Detecting Essential Metal Ions across the Tree of Life.ACS sensors · 2024Review
- A Combination of Library Screening and Rational Mutagenesis Expands the Available Color Palette of the Smallest Fluorogen-Activating Protein Tag nanoFAST.International journal of molecular sciences · 2024Article
- Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Mutant Flavin-Based Fluorescent Protein Sensors for Detecting Intracellular Zinc and Copper inACS sensors · 2022Article
- A Red Fluorescent Protein Reporter System Developed for Measuring Gene Expression in Photosynthetic Bacteria under Anaerobic Conditions.Microorganisms · 2022Article
- Enhanced small green fluorescent proteins as a multisensing platform for biosensor development.Frontiers in bioengineering and biotechnology · 2022Article
- New Orange Ligand-Dependent Fluorescent Reporter for Anaerobic Imaging.ACS chemical biology · 2021Article
- Characterization of flavin binding in oxygen-independent fluorescent reporters.AIChE journal. American Institute of Chemical Engineers · 2020Article
- TheInfection and immunity · 2020Article
- Beyond the Green Fluorescent Protein: Biomolecular Reporters for Anaerobic and Deep-Tissue Imaging.Bioconjugate chemistry · 2020Review
- Regulating vitamin B12 biosynthesis via theProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Next-Generation Fluorogen-Based Reporters and Biosensors for Advanced Bioimaging.International journal of molecular sciences · 2019Review
- An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria.Scientific reports · 2018Article
- Lighting Up Clostridium Difficile: Reporting Gene Expression Using Fluorescent Lov Domains.Scientific reports · 2016Article
- A sensitive bacterial-growth-based test reveals how intestinal Bacteroides meet their porphyrin requirement.BMC microbiology · 2015Article
- A Modified Shuttle Plasmid Facilitates Expression of a Flavin Mononucleotide-Based Fluorescent Protein in Treponema denticola ATCC 35405.Applied and environmental microbiology · 2015Article
- Article
- Fusion of a flavin-based fluorescent protein to hydroxynitrile lyase from Arabidopsis thaliana improves enzyme stability.Applied and environmental microbiology · 2013Article
- Characterization of flavin-based fluorescent proteins: an emerging class of fluorescent reporters.PloS one · 2013Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
In this study, we show the expression of flavin mononucleotide-based fluorescent protein (FbFP) BS2 as a marker for gene expression in the opportunistic human anaerobic pathogen Bacteroides fragilis. Bacteroides fragilis 638R strain carrying osu∷bs2 constructs showed inducible fluorescence following addition of maltose anaerobically compared with nonfluorescent cells under glucose-repressed conditions. Bacteria carrying ahpC∷bs2 or dps∷bs2 constructs were fluorescent following induction by oxygen compared with nonfluorescent cells from the anaerobic control cultures. In addition, when these transcriptional fusion constructs were mobilized into B. fragilis IB263, a constitutive peroxide response strain, fluorescent BS2, was detected in both anaerobic and aerobic cultures, confirming the unique properties of the FbFP BS2 to yield fluorescent signal in B. fragilis in the presence and in the absence of oxygen. Moreover, intracellular expression of BS2 was also detected when cell culture monolayers of J774.1 macrophages were incubated with B. fragilis ahpC∷bs2 or dps∷bs2 strains within an anaerobic chamber. This suggests that ahpC and dps are induced following internalization by macrophages. Thus, we show that BS2 is a suitable tool for the detection of gene expression in obligate anaerobic bacteria in in vivo studies.
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