ArticleVirologica Sinica2010
Reference gene selection for normalization of PCR analysis in chicken embryo fibroblast infected with H5N1 AIV.
Article in Virologica Sinica, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 44 citations in OpenAlex.
- Tissue-specific reference gene stability for qPCR in thermally conditioned quail embryos.Biological research · 2026Article
- Transcriptomic analysis of cecal mucosal immunity in SPF White Leghorn chicks infected with precocious and parent strains ofFrontiers in immunology · 2026Article
- Effects of glyphosate, antibiotics, and an anticoccidial drug on pancreatic gene expression and blood physiology in broilers.Journal of Zhejiang University. Science. B · 2025Article
- Elastic fibers define embryonic tissue stiffness to enable buckling morphogenesis of the small intestine.Biomaterials · 2023Article
- ELASTIC FIBERS DEFINE EMBRYONIC TISSUE STIFFNESS TO ENABLE BUCKLING MORPHOGENESIS OF THE SMALL INTESTINE.bioRxiv : the preprint server for biology · 2023Article
- Embryonic transcriptome unravels mechanisms and pathways underlying embryonic development with respect to muscle growth, egg production, and plumage formation in native and broiler chickens.Frontiers in genetics · 2022Article
- Transcriptome Analysis of Genes Responding to Infection of Leghorn Male Hepatocellular Cells With Fowl Adenovirus Serotype 4.Frontiers in veterinary science · 2022Article
- Identification of Reference Genes in Chicken Intraepithelial Lymphocyte Natural Killer Cells Infected with Very-virulent Infectious Bursal Disease Virus.Scientific reports · 2020Article
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- Selection and validation of suitable reference genes for qPCR gene expression analysis in goats and sheep under Peste des petits ruminants virus (PPRV), lineage IV infection.Scientific reports · 2018Article
- The FACT Complex Promotes Avian Leukosis Virus DNA Integration.Journal of virology · 2017Article
- The 60S ribosomal protein L13 is the most preferable reference gene to investigate gene expression in selected organs from turkeys and chickens, in context of different infection models.Veterinary research · 2016Article
- Heat Stress and Lipopolysaccharide Stimulation of Chicken Macrophage-Like Cell Line Activates Expression of Distinct Sets of Genes.PloS one · 2016Article
- A Panel of Stably Expressed Reference Genes for Real-Time qPCR Gene Expression Studies of Mallards (Anas platyrhynchos).PloS one · 2016Article
- Selection of reference genes for gene expression studies related to intramuscular fat deposition in Capra hircus skeletal muscle.PloS one · 2015Article
- Gene Expression Profiles of Chicken Embryo Fibroblasts in Response to Salmonella Enteritidis Infection.PloS one · 2015Article
- Evaluation of reference genes for quantitative real-time PCR in the brain, pituitary, and gonads of songbirds.Hormones and behavior · 2014Article
- Article
- Selection of reference genes for quantitative real-time PCR analysis in chicken embryo fibroblasts infected with avian leukosis virus subgroup J.BMC research notes · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chicken embryo fibroblasts (CEFs) are among the most commonly used cells for the study of interactions between chicken hosts and H5N1 avian influenza virus (AIV). In this study, the expression of eleven housekeeping genes typically used for the normalization of quantitative real-time PCR (QPCR) analysis in mammals were compared in CEFs infected with H5N1 AIV to determine the most reliable reference genes in this system. CEFs cultured from 10-day-old SPF chicken embryos were infected with 100 TCID(50) of H5N1 AIV and harvested at 3, 12, 24 and 30 hours post-infection. The expression levels of the eleven reference genes in infected and uninfected CEFs were determined by real-time PCR. Based on expression stability and expression levels, our data suggest that the ribosomal protein L4 (RPL4) and tyrosine 3-monooxygenase tryptophan 5-monooxygenase activation protein zeta polypeptide (YWHAZ) are the best reference genes to use in the study of host cell response to H5N1 AIV infection. However, for the study of replication levels of H5N1 AIV in CEFs, the β-actin gene (ACTB) and the ribosomal protein L4 (RPL4) gene are the best references.
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