ArticleTransgenic research2011
Strategies for selection marker-free swine transgenesis using the Sleeping Beauty transposon system.
Article in Transgenic research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 42 citations in OpenAlex.
- Accumulation of Pericardial Fat Is Associated With Alterations in Heart Rate Variability Patterns in Hypercholesterolemic Pigs.Circulation. Arrhythmia and electrophysiology · 2020Article
- Application of new biotechnologies for improvements in swine nutrition and pork production.Journal of animal science and biotechnology · 2019Review
- Changes in Skeletal Muscle and Body Weight on Sleeping Beauty Transposon-Mediated Transgenic Mice Overexpressing Pig mIGF-1.Biochemical genetics · 2018Article
- Long-term health and germline transmission in transgenic cattle following transposon-mediated gene transfer.BMC genomics · 2018Article
- Ossabaw Pigs With a PCSK9 Gain-of-Function Mutation Develop Accelerated Coronary Atherosclerotic Lesions: A Novel Model for Preclinical Studies.Journal of the American Heart Association · 2018Article
- Peripheral vascular atherosclerosis in a novel PCSK9 gain-of-function mutant Ossabaw miniature pig model.Translational research : the journal of laboratory and clinical medicine · 2018Article
- Cell-penetrating peptide-driven Cre recombination in porcine primary cells and generation of marker-free pigs.PloS one · 2018Article
- Genome-editing technologies to improve research, reproduction, and production in pigs.Molecular reproduction and development · 2017Review
- Isozygous and selectable marker-free MSTN knockout cloned pigs generated by the combined use of CRISPR/Cas9 and Cre/LoxP.Scientific reports · 2016Article
- Engineering Large Animal Species to Model Human Diseases.Current protocols in human genetics · 2016Article
- Gene targeting, genome editing: from Dolly to editors.Transgenic research · 2016Review
- Engineered Swine Models of Cancer.Frontiers in genetics · 2016Review
- Exogenous enzymes upgrade transgenesis and genetic engineering of farm animals.Cellular and molecular life sciences : CMLS · 2015Review
- Germline transgenesis in pigs by cytoplasmic microinjection of Sleeping Beauty transposons.Nature protocols · 2014Article
- Article
- Pig transgenesis by piggyBac transposition in combination with somatic cell nuclear transfer.Transgenic research · 2013Article
- Pseudo attP sites in favor of transgene integration and expression in cultured porcine cells identified by Streptomyces phage phiC31 integrase.BMC molecular biology · 2013Article
- Generation of minipigs with targeted transgene insertion by recombinase-mediated cassette exchange (RMCE) and somatic cell nuclear transfer (SCNT).Transgenic research · 2013Article
- Completion of the swine genome will simplify the production of swine as a large animal biomedical model.BMC medical genomics · 2012Article
- Efficient TALEN-mediated gene knockout in livestock.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
Abstract
Swine transgenesis by pronuclear injection or cloning has traditionally relied on illegitimate recombination of DNA into the pig genome. This often results in animals containing concatemeric arrays of transgenes that complicate characterization and can impair long-term transgene stability and expression. This is inconsistent with regulatory guidance for transgenic livestock, which also discourages the use of selection markers, particularly antibiotic resistance genes. We demonstrate that the Sleeping Beauty (SB) transposon system effectively delivers monomeric, multi-copy transgenes to the pig embryo genome by pronuclear injection without markers, as well as to donor cells for founder generation by cloning. Here we show that our method of transposon-mediated transgenesis yielded 38 cloned founder pigs that altogether harbored 100 integrants for five distinct transposons encoding either human APOBEC3G or YFP-Cre. Two strategies were employed to facilitate elimination of antibiotic genes from transgenic pigs, one based on Cre-recombinase and the other by segregation of independently transposed transgenes upon breeding.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.