ArticleArchives of virology2011
Development of a molecular-beacon-based multi-allelic real-time RT-PCR assay for the detection of human coronavirus causing severe acute respiratory syndrome (SARS-CoV): a general methodology for detecting rapidly mutating viruses.
Article in Archives of virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Optimal feature selection for COVID-19 detection with CT images enabled by metaheuristic optimization and artificial intelligence.Multimedia tools and applications · 2023Article
- Detection of Circulating SARS-CoV-2 Variants of Concern (VOCs) Using a Multiallelic Spectral Genotyping Assay.Life (Basel, Switzerland) · 2023Article
- CRISPR-based assays for rapid detection of SARS-CoV-2.Methods (San Diego, Calif.) · 2022Review
- Organ Involvement in COVID-19: A Molecular Investigation of Autopsied Patients.Microorganisms · 2022Article
- Micro/nano biomedical devices for point-of-care diagnosis of infectious respiratory diseases.Medicine in novel technology and devices · 2022Review
- An Insight Into Detection Pathways/Biosensors of Highly Infectious Coronaviruses.Molecular biotechnology · 2022Review
- A Multiallelic Molecular Beacon-Based Real-Time RT-PCR Assay for the Detection of SARS-CoV-2.Life (Basel, Switzerland) · 2021Article
- Molecular Beacon Assay Development for Severe Acute Respiratory Syndrome Coronavirus 2 Detection.Sensors (Basel, Switzerland) · 2021Article
- Electrochemical investigations for COVID-19 detection-A comparison with other viral detection methods.Chemical engineering journal (Lausanne, Switzerland : 1996) · 2021Article
- SARS-CoV, MERS-CoV and SARS-CoV-2: A Diagnostic Challenge.Measurement : journal of the International Measurement Confederation · 2021Article
- The Production of Standardized Samples with Known Concentrations for Severe Acute Respiratory Syndrome Coronavirus 2 RT-qPCR Testing Validation for Developing Countries in the Period of the Pandemic Era.BioMed research international · 2021Article
- COVID-19 reinfection: Linked Possibilities and future outlook.Journal of family medicine and primary care · 2020Review
- Recent advances in the nucleic acid-based diagnostic tool for coronavirus.Molecular biology reports · 2020Review
- Recent advances and perspectives of nucleic acid detection for coronavirus.Journal of pharmaceutical analysis · 2020Review
- Laboratory readiness and response for novel coronavirus (2019-nCoV) in expert laboratories in 30 EU/EEA countries, January 2020.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2020Article
- Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR.Archives of virology · 2017Article
- Molecular beacons: a novel optical diagnostic tool.Archivum immunologiae et therapiae experimentalis · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 6 institutions in 2 countries.
Funding
Abstract
Emerging infectious diseases have caused a global effort for development of fast and accurate detection techniques. The rapidly mutating nature of viruses presents a major difficulty, highlighting the need for specific detection of genetically diverse strains. One such infectious agent is SARS-associated coronavirus (SARS-CoV), which emerged in 2003. This study aimed to develop a real-time RT-PCR detection assay specific for SARS-CoV, taking into account its intrinsic polymorphic nature due to genetic drift and recombination and the possibility of continuous and multiple introductions of genetically non-identical strains into the human population, by using mismatch-tolerant molecular beacons designed to specifically detect the SARS-CoV S, E, M and N genes. These were applied in simple, reproducible duplex and multiplex real-time PCR assays on 25 post-mortem samples and constructed RNA controls, and they demonstrated high target detection ability and specificity. This assay can readily be adapted for detection of other emerging and rapidly mutating pathogens.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.