ArticlePlanta2011
A genomic approach to isoflavone biosynthesis in kudzu (Pueraria lobata).
Article in Planta, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 79 citations in OpenAlex.
- De novo biosynthesis ofBiodesign research · 2025Article
- Review
- Integrative Analyses of Metabolome and Transcriptome Reveal Regulatory Network of Puerarin Biosynthesis inMolecules (Basel, Switzerland) · 2024Article
- Article
- Identification and Characterization of CtUGT3 as the Key Player of Astragalin Biosynthesis inJournal of agricultural and food chemistry · 2023Article
- Comparative Transcriptome Analysis ofBiomolecules · 2023Article
- Glycosylation and methylation in the biosynthesis of isoflavonoids inFrontiers in plant science · 2023Review
- Phylogenetic Analysis and Protein Modelling of Isoflavonoid Synthase Highlights Key Catalytic Sites towards Realising New Bioengineering Endeavours.Bioengineering (Basel, Switzerland) · 2022Article
- Evaluation of pathways to thePlant direct · 2022Article
- Identification of Pueraria spp. through DNA barcoding and comparative transcriptomics.BMC plant biology · 2022Article
- Engineering the expression of plant secondary metabolites-genistein and scutellarin through an efficient transient production platform inFrontiers in plant science · 2022Article
- The Flavonoid Biosynthesis Network in Plants.International journal of molecular sciences · 2021Review
- Native and non-native host assessment towards metabolic pathway reconstructions of plant natural products.Biotechnology reports (Amsterdam, Netherlands) · 2021Review
- Article
- Metabolic Engineering of Isoflavones: An Updated Overview.Frontiers in plant science · 2021Review
- Identification of Transcription Factor Genes and Functional Characterization ofFrontiers in plant science · 2021Article
- Transcriptome sequencing and analysis reveals the molecular response to selenium stimuli inPeerJ · 2020Article
- Identification of Three (Iso)flavonoid Glucosyltransferases FromFrontiers in plant science · 2019Article
- Genome-Wide Identification ofFrontiers in plant science · 2017Article
- Involvement of three putative glucosyltransferases from the UGT72 family in flavonol glucoside/rhamnoside biosynthesis in Lotus japonicus seeds.Journal of experimental botany · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Roots of kudzu (Pueraria lobata) are a rich source of isoflavone O- and C-glycosides. Although O-glycosylation of (iso)flavonoids has been well characterized at the molecular level, no plant isoflavonoid C-glycosyltransferase genes have yet been isolated. To address the biosynthesis of kudzu isoflavonoids, we generated 6,365 high-quality expressed sequence tags (ESTs) from a subtraction cDNA library constructed using RNA from roots that differentially accumulate puerarin. The ESTs were clustered into 722 TCs and 3,913 singletons, from which 15 family I glycosyltransferases (UGTs) were identified. Hierarchical clustering analysis of the expression patterns of these UGTs with isoflavone synthase (IFS) in a range of tissues identified UGTs with potential functions in isoflavone glycosylation. The open reading frames of these UGTs were expressed in E. coli for functional analysis, and one was shown to preferentially glycosylate isoflavones at the 7-O-position. In addition, ESTs corresponding to chalcone synthase, chalcone reductase, chalcone isomerase (CHI) and 2-hydroxyisoflavanone dehydratase were identified. Recombinant CHI proteins had high activities with both 6'-deoxy- and 6'-hydroxy chalcones, typical of Type II CHIs. Establishment of this EST database and identification of genes associated with kudzu isoflavone biosynthesis and glycosylation provide a new resource for metabolic engineering of bioactive kudzu isoflavones.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.