ArticleCell research2011
Reprogramming of ovine adult fibroblasts to pluripotency via drug-inducible expression of defined factors.
Article in Cell research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
60 citing papers in PubMed, 134 citations in OpenAlex.
- Replicable generation and stable maintenance of rhesus macaque iPSCs forFrontiers in cell and developmental biology · 2026Article
- Tracing and Capturing the Epiblast Pluripotency of Sheep Preimplantation Embryos.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Advances in the Study of Pluripotent Stem Cells in Livestock.Cell proliferation · 2025Review
- Construction and Influence of Induced Pluripotent Stem Cells on Early Embryo Development in Black Bone Sheep.Biology · 2025Article
- Advancements in Stem Cell Applications for Livestock Research: A Review.Veterinary sciences · 2025Review
- Advancing stem cell technologies for conservation of wildlife biodiversity.Development (Cambridge, England) · 2024Review
- Construction of Lentiviral Vectors Carrying Six Pluripotency Genes in Yak to Obtain Yak iPSC Cells.International journal of molecular sciences · 2024Article
- The impact of induced pluripotent stem cells in animal conservation.Veterinary research communications · 2024Review
- Advances in Genetic Reprogramming: Prospects from Developmental Biology to Regenerative Medicine.Current medicinal chemistry · 2024Review
- Establishment of Capra hircus somatic cells and induction of pluripotent stem-like cells.In vitro cellular & developmental biology. Animal · 2024Article
- Reprogramming efficiency and pluripotency of mule iPSCs over its parents†.Biology of reproduction · 2023Article
- Induced pluripotent stem cells from domesticated ruminants and their potential for enhancing livestock production.Frontiers in veterinary science · 2023Review
- iPSC Technology: An Innovative Tool for Developing Clean Meat, Livestock, and Frozen Ark.Animals : an open access journal from MDPI · 2022Review
- Perspectives on scaling production of adipose tissue for food applications.Biomaterials · 2022Article
- iPSC Therapy for Myocardial Infarction in Large Animal Models: Land of Hope and Dreams.Biomedicines · 2021Review
- miR-200c-141 Enhances Sheep Kidney Cell Reprogramming into Pluripotent Cells by Targeting ZEB1.International journal of stem cells · 2021Article
- Efficient induction and sustenance of pluripotent stem cells from bovine somatic cells.Biology open · 2021Article
- The miR-302s/367 Cluster Inhibits the Proliferation and Apoptosis in Sheep Fetal Fibroblasts via the Cell Cycle and PI3K-Akt Pathways.Mammalian genome : official journal of the International Mammalian Genome Society · 2021Article
- Perspectives of pluripotent stem cells in livestock.World journal of stem cells · 2021Review
- Generation of Sheep Induced Pluripotent Stem Cells With Defined DOX-Inducible Transcription FactorsFrontiers in cell and developmental biology · 2021Article
Corrections and comments
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Authors and funding
17 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reprogramming of somatic cells in the enucleated egg made Dolly, the sheep, the first successfully cloned mammal in 1996. However, the mechanism of sheep somatic cell reprogramming has not yet been addressed. Moreover, sheep embryonic stem (ES) cells are still not available, which limits the generation of precise gene-modified sheep. In this study, we report that sheep somatic cells can be directly reprogrammed to induced pluripotent stem (iPS) cells using defined factors (Oct4, Sox2, c-Myc, Klf4, Nanog, Lin28, SV40 large T and hTERT). Our observations indicated that somatic cells from sheep are more difficult to reprogram than somatic cells from other species, in which iPS cells have been reported. We demonstrated that sheep iPS cells express ES cell markers, including alkaline phosphatase, Oct4, Nanog, Sox2, Rex1, stage-specific embryonic antigen-1, TRA-1-60, TRA-1-81 and E-cadherin. Sheep iPS cells exhibited normal karyotypes and were able to differentiate into all three germ layers both in vitro and in teratomas. Our study may help to reveal the mechanism of somatic cell reprogramming in sheep and provide a platform to explore the culture conditions for sheep ES cells. Moreover, sheep iPS cells may be directly used to generate precise gene-modified sheep.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.