ArticlePlant physiology2011
Cloning and characterization of purple acid phosphatase phytases from wheat, barley, maize, and rice.
Article in Plant physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 117 citations in OpenAlex.
- Systematic characterization of purple acid phosphatases provides targets for improving phosphorus use efficiency in cotton.Functional & integrative genomics · 2026Article
- Transgenic barley over-expressingGM crops & food · 2025Article
- Analysis and profiling of the purple acid phosphatase gene family in wheat (Triticum aestivum L.).Protoplasma · 2025Article
- Optimized barley phytase gene expression by focused FIND-IT screening for mutations inFrontiers in plant science · 2024Article
- Structural and functional profile of phytases across the domains of life.Current research in structural biology · 2024Review
- The Impact of Various Organic Phosphorus Carriers on the Uptake and Use Efficiency in Barley.International journal of molecular sciences · 2023Article
- Enhancing Phytate Availability in Soils and Phytate-P Acquisition by Plants: A Review.Environmental science & technology · 2022Review
- Structure of a cereal purple acid phytase provides new insights to phytate degradation in plants.Plant communications · 2022Article
- Purple acid phosphatases: roles in phosphate utilization and new emerging functions.Plant cell reports · 2022Review
- Barley Nepenthesin-Like Aspartic ProteaseFrontiers in plant science · 2021Article
- Identification of individual components of a commercial wheat germ acid phosphatase preparation.PloS one · 2021Article
- Biofortification and bioavailability of Zn, Fe and Se in wheat: present status and future prospects.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Review
- Identification, Association of Natural Variation and Expression Analysis ofPlants (Basel, Switzerland) · 2020Article
- Globoids and Phytase: The Mineral Storage and Release System in Seeds.International journal of molecular sciences · 2020Review
- Horizontal Stacking ofFrontiers in plant science · 2020Article
- Characterization of Purple Acid Phosphatase Family and Functional Analysis ofFrontiers in plant science · 2020Article
- Genetic transformation of tropical maize (3 Biotech · 2019Article
- Molecular Advances on Phytases in Barley and Wheat.International journal of molecular sciences · 2019Review
- Identification of Tea Plant Purple Acid Phosphatase Genes and Their Expression Responses to Excess Iron.International journal of molecular sciences · 2019Article
- ZmAPRG, an uncharacterized gene, enhances acid phosphatase activity and Pi concentration in maize leaf during phosphate starvation.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Barley (Hordeum vulgare) and wheat (Triticum aestivum) possess significant phytase activity in the mature grains. Maize (Zea mays) and rice (Oryza sativa) possess little or virtually no preformed phytase activity in the mature grain and depend fully on de novo synthesis during germination. Here, it is demonstrated that wheat, barley, maize, and rice all possess purple acid phosphatase (PAP) genes that, expressed in Pichia pastoris, give fully functional phytases (PAPhys) with very similar enzyme kinetics. Preformed wheat PAPhy was localized to the protein crystalloid of the aleurone vacuole. Phylogenetic analyses indicated that PAPhys possess four conserved domains unique to the PAPhys. In barley and wheat, the PAPhy genes can be grouped as PAPhy_a or PAPhy_b isogenes (barley, HvPAPhy_a, HvPAPhy_b1, and HvPAPhy_b2; wheat, TaPAPhy_a1, TaPAPhy_a2, TaPAPhy_b1, and TaPAPhy_b2). In rice and maize, only the b type (OsPAPhy_b and ZmPAPhy_b, respectively) were identified. HvPAPhy_a and HvPAPhy_b1/b2 share 86% and TaPAPhya1/a2 and TaPAPhyb1/b2 share up to 90% (TaPAPhy_a2 and TaPAPhy_b2) identical amino acid sequences. despite of this, PAPhy_a and PAPhy_b isogenes are differentially expressed during grain development and germination. In wheat, it was demonstrated that a and b isogene expression is driven by different promoters (approximately 31% identity). TaPAPhy_a/b promoter reporter gene expression in transgenic grains and peptide mapping of TaPAPhy purified from wheat bran and germinating grains confirmed that the PAPhy_a isogene set present in wheat/barley but not in rice/maize is the origin of high phytase activity in mature grains.
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