ArticleProceedings of the National Academy of Sciences of the United States of America2011
Atomic model of a cypovirus built from cryo-EM structure provides insight into the mechanism of mRNA capping.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 69 citations in OpenAlex.
- RNA Virus Discovery Sheds Light on the Virome of a Major Vineyard Pest, the European Grapevine Moth (Viruses · 2025Article
- Integration of Cypoviruses into polyhedrin matrix.Nanoscale advances · 2023Article
- In situ structures of polymerase complex of mammalian reovirus illuminate RdRp activation and transcription regulation.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- AlphaFold2 and CryoEM: Revisiting CryoEM modeling in near-atomic resolution density maps.iScience · 2022Article
- Multiple conformations of trimeric spikes visualized on a non-enveloped virus.Nature communications · 2022Article
- Viral Capsid and Polymerase in Reoviridae.Sub-cellular biochemistry · 2022Article
- Cryo-EM reveals a previously unrecognized structural protein of a dsRNA virus implicated in its extracellular transmission.PLoS pathogens · 2021Article
- Captivating Perplexities ofViruses · 2021Review
- Structure of RNA polymerase complex and genome within a dsRNA virus provides insights into the mechanisms of transcription and assembly.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Real-space refinement in PHENIX for cryo-EM and crystallography.Acta crystallographica. Section D, Structural biology · 2018Article
- Exogenous gene can be expressed by a recombinant Bombyx mori cypovirus.Applied microbiology and biotechnology · 2018Article
- Identification and characterization of vp7 gene in Bombyx mori cytoplasmic polyhedrosis virus.Gene · 2017Article
- Cypovirus capsid protein VP5 has nucleoside triphosphatase activity.Virologica Sinica · 2017Article
- Article
- MTase Domain of Dendrolimus punctatus cypovirus VP3 Mediates Virion Attachment and Interacts with Host ALP Protein.Viruses · 2017Article
- Biological cryo-electron microscopy in China.Protein science : a publication of the Protein Society · 2017Review
- Constrained Cyclic Coordinate Descent for Cryo-EM Images at Medium Resolutions: Beyond the Protein Loop Closure Problem.Robotica · 2016Article
- Symmetry-mismatch reconstruction of genomes and associated proteins within icosahedral viruses using cryo-EM.Biophysics reports · 2016Article
- Exploring the inside details of virions by electron microscopy.Biophysics reports · 2016Article
- Tools for macromolecular model building and refinement into electron cryo-microscopy reconstructions.Acta crystallographica. Section D, Biological crystallography · 2015Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cytoplasmic polyhedrosis virus (CPV) from the family Reoviridae belongs to a subgroup of "turreted" reoviruses, in which the mRNA capping activity occurs in a pentameric turret. We report a full atomic model of CPV built from a 3D density map obtained using cryoelectron microscopy. The image data for the 3D reconstruction were acquired exclusively from a CCD camera. Our structure shows that the enzymatic domains of the pentameric turret of CPV are topologically conserved and that there are five unique channels connecting the guanylyltransferase and methyltransferase regions. This structural organization reveals how the channels guide nascent mRNA sequentially to guanylyltransferase, 7-N-methyltransferase, and 2'-O-methyltransferase in the turret, undergoing the highly coordinated mRNA capping activity. Furthermore, by fitting the deduced amino acid sequence of the protein VP5 to 120 large protrusion proteins on the CPV capsid shell, we confirmed that this protrusion protein is encoded by CPV RNA segment 7.
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