ArticleMolecular microbiology2011
Deletion of the RluD pseudouridine synthase promotes SsrA peptide tagging of ribosomal protein S7.
Article in Molecular microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 16 citations in OpenAlex.
- Compendium of RNA modifications for bacterial stress adaptation.Microbiology and molecular biology reviews : MMBR · 2026Review
- Suppressor analysis links trans-translation and ribosomal protein uS7 to RluD function in Escherichia coli.Biochemical and biophysical research communications · 2024Article
- Laboratory strains ofMicrobial genomics · 2023Article
- The impact of genetic diversity on gene essentiality within the Escherichia coli species.Nature microbiology · 2021Article
- Pseudouridine-Free Escherichia coli Ribosomes.Journal of bacteriology · 2018Article
- Interactions of release factor RF3 with the translation machinery.Molecular genetics and genomics : MGG · 2015Article
- Release of nonstop ribosomes is essential.mBio · 2014Article
- Adaptation of Escherichia coli to elevated sodium concentrations increases cation tolerance and enables greater lactic acid production.Applied and environmental microbiology · 2014Article
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2 authors at 2 institutions in 2 countries.
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Abstract
RluD catalyses formation of three pseudouridine residues within helix 69 of the 50S ribosome subunit. Helix 69 makes important contacts with the decoding centre on the 30S subunit and deletion of rluD was recently shown to interfere with translation termination in Escherichia coli. Here, we show that deletion of rluD increases tmRNA activity on ribosomes undergoing release factor 2 (RF2)-mediated termination at UGA stop codons. Strikingly, tmRNA-mediated SsrA peptide tagging of two proteins, ribosomal protein S7 and LacI, was dramatically increased in ΔrluD cells. S7 tagging was due to a unique C-terminal peptide extension found in E. coli K-12 strains. Introduction of the rpsG gene (encoding S7) from an E. coli B strain abrogated S7 tagging in the ΔrluD background, and partially complemented the mutant's slow-growth phenotype. Additionally, exchange of the K-12 prfB gene (encoding RF2) with the B strain allele greatly reduced tagging in ΔrluD cells. In contrast to E. coli K-12 cells, deletion of rluD in an E. coli B strain resulted in no growth phenotype. These findings indicate that the originally observed rluD phenotypes result from synthetic interactions with rpsG and prfB alleles found within E. coli K-12 strains.
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