ArticleFEMS immunology and medical microbiology2011
Shigella enterotoxin-2 is a type III effector that participates in Shigella-induced interleukin 8 secretion by epithelial cells.
Article in FEMS immunology and medical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 58 citations in OpenAlex.
- Virulence factors associated with pediatric shigellosis in Brazilian Amazon.BioMed research international · 2014Trial
- Molecular Mechanisms ofInternational journal of molecular sciences · 2023Review
- MP4: a machine learning based classification tool for prediction and functional annotation of pathogenic proteins from metagenomic and genomic datasets.BMC bioinformatics · 2022Article
- Convergence of virulence and resistance in international clones of WHO critical priority enterobacterales isolated from Marine Bivalves.Scientific reports · 2022Article
- Virulence factors and molecular characteristics of Shigella flexneri isolated from calves with diarrhea.BMC microbiology · 2021Article
- Enteropathogenic Infections: Organoids Go Bacterial.Stem cells international · 2021Review
- Staying out or Going in? The Interplay between Type 3 and Type 5 Secretion Systems in Adhesion and Invasion of Enterobacterial Pathogens.International journal of molecular sciences · 2020Review
- Maintenance of the virulence plasmid in Shigella flexneri is influenced by Lon and two functional partitioning systems.Molecular microbiology · 2019Article
- Transposon mutagenesis and identification of mutated genes in growth-delayed Edwardsiella ictaluri.BMC microbiology · 2019Article
- Plasmid-Mediated Quinolone Resistance inFrontiers in microbiology · 2018Article
- Profiling of Virulence-associated Factors inOsong public health and research perspectives · 2017Article
- How Do the Virulence Factors ofFrontiers in cellular and infection microbiology · 2017Review
- The synthesis of OspD3 (ShET2) in Shigella flexneri is independent of OspC1.Gut microbes · 2016Article
- Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing.Journal of visualized experiments : JoVE · 2016Article
- Occurrence of genes encoding enterotoxins in uropathogenic Escherichia coli isolates.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2015Article
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- Recent advances in understanding enteric pathogenic Escherichia coli.Clinical microbiology reviews · 2013Review
- Pathogenesis of human enterovirulent bacteria: lessons from cultured, fully differentiated human colon cancer cell lines.Microbiology and molecular biology reviews : MMBR · 2013Review
- Progress and pitfalls in Shigella vaccine research.Nature reviews. Gastroenterology & hepatology · 2013Review
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
We have previously described a protein termed Shigella enterotoxin 2 (ShET-2), which induces rises in short-circuit current in rabbit ileum mounted in the Ussing chamber. Published reports have postulated that ShET-2 may be secreted by the Shigella type III secretion system (T3SS). In this study, we show that ShET-2 secretion into the extracellular space requires the T3SS in Shigella flexneri 2a strain 2457T and a ShET-2-TEM fusion was translocated into epithelial cells in a T3SS-dependent manner. The ShET-2 gene, sen, is encoded downstream of the ospC1 gene of S. flexneri, and we show that sen is cotranscribed with this T3SS-secreted product. Considering that T3SS effectors have diverse roles in Shigella infection and that vaccine constructs lacking ShET-2 are attenuated in volunteers, we asked whether ShET-2 has a function other than its enterotoxic activity. We constructed a ShET-2 mutant in 2457T and tested its effect on epithelial cell invasion, plaque formation, guinea pig keratoconjunctivitis and interleukin 8 (IL-8) secretion from infected monolayers. Although other phenotypes were not different compared with the wild-type parent, we found that HEp-2 and T84 cells infected with the ShET-2 mutant exhibited significantly reduced IL-8 secretion into the basolateral compartment, suggesting that ShET-2 might participate in the Shigella-induced inflammation of epithelial cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.