ArticleACS chemical biology2011
Dual wavelength photoactivation of cAMP- and cGMP-dependent protein kinase signaling pathways.
Article in ACS chemical biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 53 citations in OpenAlex.
- Smoothened and ciliary GPCRs regulate ciliary protein kinase A activity involved in Hedgehog signal transduction.PLoS biology · 2026Article
- Photocleavable Systems for Cell Biology: Conceptual Design across Molecular Modalities.Chembiochem : a European journal of chemical biology · 2025Review
- Phototriggered structures: Latest advances in biomedical applications.Acta pharmaceutica Sinica. B · 2023Review
- Review
- Visible-to-NIR-Light Activated Release: From Small Molecules to Nanomaterials.Chemical reviews · 2020Article
- Optical control of protein phosphatase function.Nature communications · 2019Article
- Reversible Control of Protein Localization in Living Cells Using a Photocaged-Photocleavable Chemical Dimerizer.Journal of the American Chemical Society · 2018Article
- Optochemical Control of Biological Processes in Cells and Animals.Angewandte Chemie (International ed. in English) · 2018Review
- Design and Profiling of a Subcellular Targeted Optogenetic cAMP-Dependent Protein Kinase.Cell chemical biology · 2018Article
- An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation.Journal of visualized experiments : JoVE · 2017Article
- Two-color, one-photon uncaging of glutamate and GABA.PloS one · 2017Article
- Orthogonal photoswitching in a multifunctional molecular system.Nature communications · 2016Article
- Vitamin B12: a tunable, long wavelength, light-responsive platform for launching therapeutic agents.Accounts of chemical research · 2015Article
- Chronic nicotine treatment enhances vascular smooth muscle relaxation in rats.Acta pharmacologica Sinica · 2015Article
- Caged compounds for multichromic optical interrogation of neural systems.The European journal of neuroscience · 2015Article
- Sequential gene silencing using wavelength-selective caged morpholino oligonucleotides.Angewandte Chemie (International ed. in English) · 2014Article
- How to control proteins with light in living systems.Nature chemical biology · 2014Review
- Optochemical control of deoxyoligonucleotide function via a nucleobase-caging approach.Accounts of chemical research · 2014Article
- Tunable visible and near-IR photoactivation of light-responsive compounds by using fluorophores as light-capturing antennas.Angewandte Chemie (International ed. in English) · 2014Article
- Spectral evolution of a photochemical protecting group for orthogonal two-color uncaging with visible light.Journal of the American Chemical Society · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The spatial and temporal organization of biological systems offers a level of complexity that is challenging to probe with conventional reagents. Photoactivatable (caged) compounds represent one strategy by which spatiotemporal organizational complexities can be addressed. However, since the vast majority of caged species are triggered by UV light, it is not feasible to orthogonally control two or more spatiotemporal elements of the phenomenon under investigation. For example, the cGMP- and cAMP-dependent protein kinases are highly homologous enzymes, separated in time and space, which mediate the phosphorylation of both distinct and common protein substrates. However, current technology is unable to discriminate, in a temporally or spatially selective fashion, between these enzymes and/or the pathways they influence. We describe herein the intracellular triggering of a cGMP-mediated pathway with 360 nm light and the corresponding cAMP-mediated pathway with 440 nm light. Dual wavelength photoactivation was assessed in A10 cells by monitoring the phosphorylation of vasodilator-stimulated phosphoprotein (VASP), a known substrate for both the cAMP- and cGMP-dependent protein kinases. Illumination at 440 nm elicits a cAMP-dependent phosphorylation of VASP at Ser157, whereas 360 nm exposure triggers the phosphorylation of both Ser157 and Ser239. This is the first example of wavelength-distinct activation of two separate nodes of a common signaling pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.