ArticlePloS one2010
Nε-lysine acetylation of a bacterial transcription factor inhibits Its DNA-binding activity.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 97 papers.
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Who cites it
97 citing papers in PubMed, 143 citations in OpenAlex.
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- Inhibition of Histone Lysine Acetyltransferases by Coenzyme A Analogs.Molecules (Basel, Switzerland) · 2026Article
- The two-component system CpxA/CpxR regulates pathogenesis and stress adaptability in the poplar canker bacterium Lonsdalea populi.Molecular plant pathology · 2024Article
- Niacinamide Antimicrobial Efficacy and Its Mode of Action via Microbial Cell Cycle Arrest.Microorganisms · 2024Article
- Bacteria employ lysine acetylation of transcriptional regulators to adapt gene expression to cellular metabolism.Nature communications · 2024Article
- Acetylomics reveals an extensive acetylation diversity withinmicroLife · 2024Article
- Acetylation of K188 and K192 inhibits the DNA-binding ability of NarL to regulateApplied and environmental microbiology · 2023Article
- Insights into the Lysine Acetylome of the HaloarchaeonAntioxidants (Basel, Switzerland) · 2023Article
- Systematic Analysis of Lysine Acetylation Reveals Diverse Functions in Azorhizobium caulinodans Strain ORS571.Microbiology spectrum · 2023Article
- Acetylation of NarL K188 and K192 is involved in regulating Escherichia coli anaerobic nitrate respiration.Applied microbiology and biotechnology · 2022Article
- Acetylation of Lactate Dehydrogenase Negatively Regulates the Acidogenicity of Streptococcus mutans.mBio · 2022Article
- Contribution of NmSystems · 2021Article
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- Structure-based analyses of Salmonella RcsB variants unravel new features of the Rcs regulon.Nucleic acids research · 2021Article
- Bacterial Sirtuins Overview: An Open Niche to Explore.Frontiers in microbiology · 2021Review
- Post-translational Lysine Ac(et)ylation in Bacteria: A Biochemical, Structural, and Synthetic Biological Perspective.Frontiers in microbiology · 2021Review
- Regulation of gene expression by protein lysine acetylation in Salmonella.Journal of microbiology (Seoul, Korea) · 2020Review
- Protein acetylation and deacetylation: An important regulatory modification in gene transcription (Review).Experimental and therapeutic medicine · 2020Review
- The Phage-EncodedViruses · 2020Article
- Article
37 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Evidence suggesting that eukaryotes and archaea use reversible N(ε)-lysine (N(ε)-Lys) acetylation to modulate gene expression has been reported, but evidence for bacterial use of N(ε)-Lys acetylation for this purpose is lacking. Here, we report data in support of the notion that bacteria can control gene expression by modulating the acetylation state of transcription factors (TFs). We screened the E. coli proteome for substrates of the bacterial Gcn5-like protein acetyltransferase (Pat). Pat acetylated four TFs, including the RcsB global regulatory protein, which controls cell division, and capsule and flagellum biosynthesis in many bacteria. Pat acetylated residue Lys180 of RcsB, and the NAD(+)-dependent Sir2 (sirtuin)-like protein deacetylase (CobB) deacetylated acetylated RcsB (RcsB(Ac)), demonstrating that N(ε)-Lys acetylation of RcsB is reversible. Analysis of RcsB(Ac) and variant RcsB proteins carrying substitutions at Lys180 provided biochemical and physiological evidence implicating Lys180 as a critical residue for RcsB DNA-binding activity. These findings further the likelihood that reversible N(ε)-Lys acetylation of transcription factors is a mode of regulation of gene expression used by all cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.