ArticleNature methods2011
A photoprotection strategy for microsecond-resolution single-molecule fluorescence spectroscopy.
Article in Nature methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
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- An optofluidic antenna for enhancing the sensitivity of single-emitter measurements.Nature communications · 2024Article
- Simple and Efficient Detection Scheme of Two-Color Fluorescence Correlation Spectroscopy for Protein Dynamics Investigation from Nanoseconds to Milliseconds.ACS physical chemistry Au · 2024Article
- Trends in Single-Molecule Total Internal Reflection Fluorescence Imaging and Their Biological Applications with Lab-on-a-Chip Technology.Sensors (Basel, Switzerland) · 2023Review
- DNA Origami Nanoantennas for Fluorescence Enhancement.Accounts of chemical research · 2021Article
- Novel voltammetric tumor necrosis factor-alpha (TNF-α) immunosensor based on gold nanoparticles involved in thiol-functionalized multi-walled carbon nanotubes and bimetallic Ni/Cu-MOFs.Analytical and bioanalytical chemistry · 2021Article
- FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices.eLife · 2021Review
- Ligand modulation of the conformational dynamics of the AScientific reports · 2021Article
- Downhill, Ultrafast and Fast Folding Proteins Revised.International journal of molecular sciences · 2020Article
- Tuning the Baird aromatic triplet-state energy of cyclooctatetraene to maximize the self-healing mechanism in organic fluorophores.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Unifying Mechanism for Thiol-Induced Photoswitching and Photostability of Cyanine Dyes.Journal of the American Chemical Society · 2020Article
- Ratiometric Single-Molecule FRET Measurements to Probe Conformational Subpopulations of Intrinsically Disordered Proteins.Current protocols in chemical biology · 2020Article
- Diffusion-limited association of disordered protein by non-native electrostatic interactions.Nature communications · 2018Article
- Investigating supramolecular systems using Förster resonance energy transfer.Chemical Society reviews · 2018Review
- Expression and purification of nuclease-free protocatechuate 3,4-dioxygenase for prolonged single-molecule fluorescence imaging.Analytical biochemistry · 2018Article
- Protein folding transition path times from single molecule FRET.Current opinion in structural biology · 2018Review
- Characterization of Fluorescein Arsenical Hairpin (FlAsH) as a Probe for Single-Molecule Fluorescence Spectroscopy.Scientific reports · 2017Article
- Substrate Oxide Layer Thickness Optimization for a Dual-Width Plasmonic Grating for Surface-Enhanced Raman Spectroscopy (SERS) Biosensor Applications.Sensors (Basel, Switzerland) · 2017Article
- Evidence for the principle of minimal frustration in the evolution of protein folding landscapes.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Time resolution of current single-molecule fluorescence techniques is limited to milliseconds because of dye blinking and bleaching. Here we introduce a photoprotection strategy that affords microsecond resolution by combining efficient triplet quenching by oxygen and Trolox with minimized bleaching via the oxygen radical scavenger cysteamine. Using this approach we resolved the single-molecule microsecond conformational fluctuations of two proteins: the two-state folder α-spectrin SH3 domain and the ultrafast downhill folder BBL.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.