ArticleThe Journal of cell biology1990
Synaptophysin binds to physophilin, a putative synaptic plasma membrane protein.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 81 citations in OpenAlex.
- Novel excitation-contraction coupling related genes reveal aspects of muscle weakness beyond atrophy-new hopes for treatment of musculoskeletal diseases.Frontiers in physiology · 2014Review
- Vesicle-associated membrane protein 4 is implicated in trans-Golgi network vesicle trafficking.Molecular biology of the cell · 1999Article
- Acetylcholine release and the cholinergic genomic locus.Molecular neurobiology · 1998Review
- Synaptobrevin binding to synaptophysin: a potential mechanism for controlling the exocytotic fusion machine.The EMBO journal · 1995Article
- The synaptic vesicle proteins SV2, synaptotagmin and synaptophysin are sorted to separate cellular compartments in CHO fibroblasts.The Journal of cell biology · 1993Article
- Regulated exocytosis.The Biochemical journal · 1993Review
- Annexin II is a major component of fusogenic endosomal vesicles.The Journal of cell biology · 1993Article
- Mechanisms in the regulation of neurotransmitter release from brain nerve terminals: current hypotheses.Neurochemical research · 1993Review
- Amphiphysin, a novel protein associated with synaptic vesicles.The EMBO journal · 1992Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed procedures for detecting synaptic vesicle-binding proteins by using glutaraldehyde-fixed or native vesicle fractions as absorbent matrices. Both adsorbents identify a prominent synaptic vesicle-binding protein of 36 kD in rat brain synaptosomes and mouse brain primary cultures. The binding of this protein to synaptic vesicles is competed by synaptophysin, a major integral membrane protein of synaptic vesicles, with half-maximal inhibition seen between 10(-8) and 10(-7) M synaptophysin. Because of its affinity for synaptophysin, we named the 36-kD synaptic vesicle-binding protein physophilin (psi nu sigma alpha, greek = bubble, vesicle; psi iota lambda os, greek = friend). Physophilin exhibits an isoelectric point of approximately 7.8, a Stokes radius of 6.6 nm, and an apparent sedimentation coefficient of 5.6 S, pointing to an oligomeric structure of this protein. It is present in synaptic plasma membranes prepared from synaptosomes but not in synaptic vesicles. In solubilization experiments, physophilin behaves as an integral membrane protein. Thus, a putative synaptic plasma membrane protein exhibits a specific interaction with one of the major membrane proteins of synaptic vesicles. This interaction may play a role in docking and/or fusion of synaptic vesicles to the presynaptic plasma membrane.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.