ArticleJournal of bacteriology2011
Inactivation of σE and σG in Clostridium acetobutylicum illuminates their roles in clostridial-cell-form biogenesis, granulose synthesis, solventogenesis, and spore morphogenesis.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 78 citations in OpenAlex.
- Genetic tools for the electrotrophApplied and environmental microbiology · 2024Article
- Development of a genetic engineering toolbox for syngas-utilizing acetogen Clostridium sp. AWRP.Microbial cell factories · 2024Article
- The Lanthipeptide Synthetase-like Protein CA_C0082 Is an Effector of Agr Quorum Sensing inMicroorganisms · 2023Article
- Genome-Wide TSS Distribution in Three Related Clostridia with Normalized Capp-Switch Sequencing.Microbiology spectrum · 2022Article
- Sporulation in solventogenic and acetogenic clostridia.Applied microbiology and biotechnology · 2021Review
- Towards continuous industrial bioprocessing with solventogenic and acetogenic clostridia: challenges, progress and perspectives.Journal of industrial microbiology & biotechnology · 2020Review
- Development of Strong Anaerobic Fluorescent Reporters for Clostridium acetobutylicum and Clostridium ljungdahlii Using HaloTag and SNAP-tag Proteins.Applied and environmental microbiology · 2020Article
- Article
- Development of optimal steam explosion pretreatment and highly effective cell factory for bioconversion of grain vinegar residue to butanol.Biotechnology for biofuels · 2020Article
- Transcriptomic and Phenotypic Analysis of aFrontiers in microbiology · 2020Article
- Pathway dissection, regulation, engineering and application: lessons learned from biobutanol production by solventogenic clostridia.Biotechnology for biofuels · 2020Review
- σScientific reports · 2019Article
- Small and Low but Potent: the Complex Regulatory Role of the Small RNA SolB in Solventogenesis in Clostridium acetobutylicum.Applied and environmental microbiology · 2018Article
- Genome and transcriptome of the natural isopropanol producer Clostridium beijerinckii DSM6423.BMC genomics · 2018Article
- Functional Expression of the Clostridium ljungdahlii Acetyl-Coenzyme A Synthase in Clostridium acetobutylicum as Demonstrated by a NovelApplied and environmental microbiology · 2018Article
- The industrial anaerobe Clostridium acetobutylicum uses polyketides to regulate cellular differentiation.Nature communications · 2017Article
- Development of a High-Efficiency Transformation Method and Implementation of Rational Metabolic Engineering for the Industrial Butanol Hyperproducer Clostridium saccharoperbutylacetonicum Strain N1-4.Applied and environmental microbiology · 2017Article
- Chemical and Stress Resistances ofFrontiers in microbiology · 2016Article
- A Quantitative System-Scale Characterization of the Metabolism of Clostridium acetobutylicum.mBio · 2015Article
- Diverse mechanisms regulate sporulation sigma factor activity in the Firmicutes.Current opinion in microbiology · 2015Review
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
Central to all clostridia is the orchestration of endospore formation (i.e., sporulation) and, specifically, the roles of differentiation-associated sigma factors. Moreover, there is considerable applied interest in understanding the roles of these sigma factors in other stationary-phase phenomena, such as solvent production (i.e., solventogenesis). Here we separately inactivated by gene disruption the major sporulation-specific sigma factors, σ(E) and σ(G), and performed an initial analysis to elucidate their roles in sporulation-related morphogenesis and solventogenesis in Clostridium acetobutylicum. The terminal differentiation phenotype for the sigE inactivation mutant stalled in sporulation prior to asymmetric septum formation, appeared vegetative-like often with an accumulation of DNA at both poles, frequently exhibited two longitudinal internal membranes, and did not synthesize granulose. The sigE inactivation mutant did produce the characteristic solvents (i.e., butanol and acetone), but the extent of solventogenesis was dependent on the physiological state of the inoculum. The sigG inactivation mutant stalled in sporulation during endospore maturation, exhibiting engulfment and partial cortex and spore coat formation. Lastly, the sigG inactivation mutant did produce granulose and exhibited wild-type-like solventogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.