ArticleApplied and environmental microbiology2011
Identification of cold-temperature-regulated genes in Flavobacterium psychrophilum.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
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- Identification of a novel type II-C Cas9 from the fish pathogenFrontiers in microbiology · 2023Article
- Transcriptomic time-series analysis of cold- and heat-shock response in psychrotrophic lactic acid bacteria.BMC genomics · 2021Article
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- Reference genes for real-time RT-PCR expression studies in an Antarctic Pseudomonas exposed to different temperature conditions.Extremophiles : life under extreme conditions · 2019Article
- Temperature-Dependent Gene Expression inFrontiers in microbiology · 2018Article
- Stress Tolerance-Related Genetic Traits of Fish PathogenFrontiers in microbiology · 2018Article
- Comparative Genomics and Transcriptional Analysis ofFrontiers in microbiology · 2017Article
- Different Phenotypes of Mature Biofilm inFrontiers in cellular and infection microbiology · 2017Article
- Characterization of a temperature-responsive two component regulatory system from the Antarctic archaeon, Methanococcoides burtonii.Scientific reports · 2016Article
- Draft Genome Sequences of Three Flavobacterium psychrophilum Strains Isolated from Coldwater Disease Outbreaks at Three Production Hatcheries.Genome announcements · 2016Article
- Comparative Genome Analysis Provides Insights into the Pathogenicity of Flavobacterium psychrophilum.PloS one · 2016Article
- Temperature-dependent expression of virulence genes in fish-pathogenic bacteria.Frontiers in microbiology · 2015Review
- The CLO3403/CLO3404 two-component system of Clostridium botulinum E1 Beluga is important for cold shock response and growth at low temperatures.Applied and environmental microbiology · 2014Article
- Genome-based comparative analyses of Antarctic and temperate species of Paenibacillus.PloS one · 2014Article
- Involvement of two-component system CBO0366/CBO0365 in the cold shock response and growth of group I (proteolytic) Clostridium botulinum ATCC 3502 at low temperatures.Applied and environmental microbiology · 2012Article
- Bacterial gene expression at low temperatures.Extremophiles : life under extreme conditions · 2012Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Flavobacterium psychrophilum is the etiological agent of bacterial coldwater disease (BCWD) and rainbow trout fry syndrome (RTFS). It causes disease primarily in fresh water-reared salmonids, but other fish species can also be affected. A diverse array of clinical conditions is associated with BCWD, including tail rot (peduncle disease), necrotic myositis, and cephalic osteochondritis. Degradation of connective and muscular tissues by extracellular proteases is common to all of these presentations. There are no effective vaccines to prevent BCWD or RTFS, and antibiotics are often used to prevent and control disease. To identify virulence factors that might permit development of an efficacious vaccine, cDNA suppression subtractive hybridization (SSH) was used to identify cold-regulated genes in a virulent strain of F. psychrophilum. Genes predicted to encode a two-component system sensor histidine kinase (LytS), an ATP-dependent RNA helicase, a multidrug ABC transporter permease/ATPase, an outer membrane protein/protective antigen OMA87, an M43 cytophagalysin zinc-dependent metalloprotease, a hypothetical protein, and four housekeeping genes were upregulated at 8°C versus the level of expression at 20°C. Because no F. psychrophilum gene was known to be suitable as an internal standard in reverse transcription-quantitative real-time PCR (RT-qPCR) experiments, the expression stability of nine commonly used reference genes was evaluated at 8°C and 20°C. Expression of the 16S rRNA was equivalent at both temperatures, and this gene was used in RT-qPCR experiments to verify the SSH findings. With the exception of the ATCC 49513 strain, similar patterns of gene expression were obtained with 11 other representative strains of F. psychrophilum.
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Registered trials
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