ArticleJournal of dentistry2011
Immunohistochemical and biochemical assay of MMP-3 in human dentine.
Article in Journal of dentistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it, 96 citations in OpenAlex.
- Mechanisms regulating the degradation of dentin matrices by endogenous dentin proteases and their role in dental adhesion. A review.American journal of dentistry · 2014Pooled it
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- Effects of Etching Time and Ethanol Wet Bonding on Bond Strength and Metalloproteinase Activity in Radicular Dentin.Journal of clinical medicine · 2024Article
- Targeting Matrix Metalloproteinase-3 for Dental Caries Prevention Using Herbal Isolates: MMP3 Inhibition by Cinnamic Acids.International journal of dentistry · 2024Article
- Dentin Degradation: From Tissue Breakdown to Possibilities for Therapeutic Intervention.Current oral health reports · 2023Article
- Effect of Endodontic Irrigating Solutions on Radicular Dentine Structure and Matrix Metalloproteinases-A Comprehensive Review.Dentistry journal · 2022Review
- Exploiting Dentine Matrix Proteins in Cell-Free Approaches for Periradicular Tissue Engineering.Tissue engineering. Part B, Reviews · 2022Review
- Expression of matrix metalloproteinase-3 and -10 is up-regulated in the periodontal tissues of aged mice.Journal of periodontal research · 2022Article
- The Influence of Different Bleaching Protocols on Dentinal Enzymatic Activity: An In Vitro Study.Molecules (Basel, Switzerland) · 2022Article
- Captopril inhibits matrix metalloproteinase activity and improves dentin bonding durability.Clinical oral investigations · 2022Article
- Biochemical and immunohistochemical analysis of tissue inhibitor of metalloproteinases-1 in human sound dentin.Clinical oral investigations · 2021Article
- Matrix metalloproteinases gene variants and dental caries in Czech children.BMC oral health · 2020Article
- Effects of Er:YAG and Diode Laser Irradiation on Dental Pulp Cells and Tissues.International journal of molecular sciences · 2018Article
- Effect of zinc on the collagen degradation in acid-etched dentin.Journal of dental sciences · 2018Article
- The dynamics of TGF-β in dental pulp, odontoblasts and dentin.Scientific reports · 2018Article
- Recent Advances in Adhesive Bonding - The Role of Biomolecules, Nanocompounds, and Bonding Strategies in Enhancing Resin Bonding to Dental Substrates.Current oral health reports · 2017Article
- Effect of ultraviolet A-induced crosslinking on dentin collagen matrix.Dental materials : official publication of the Academy of Dental Materials · 2015Article
- Effect of Sodium Fluoride on the endogenous MMP Activity of Dentin Matrices.Journal of nature and science · 2015Article
- Role of dentin MMPs in caries progression and bond stability.Journal of dental research · 2015Review
Corrections and comments
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Authors and funding
11 authors at 8 institutions in 4 countries.
Funding
Abstract
objectiveThe function of endogenous MMP-3 and its distribution within the human dentine is unclear. Thus, the aim of the present study was to assay the presence and distribution of MMP-3 within human sound dentine by means of biochemical and immunohistochemical assays.
methodsPowdered dentine from extracted human teeth was prepared and (1) partially demineralised with 1% H(3)PO(4) for 10min or (2) untreated (control). The presence of MMP-3 was measured using a colorimetric assay system (QuantiSir™, Epigentek, USA). Additional cryo-fractured dentine fragments were processed for immunohistochemical identification of MMP-3 under FEI-SEM. Casein-zymography was used to investigate MMP-3 activity.
resultsMMP-3 detected level was 2.732ng/μL in partially demineralised dentine powder, whilst it increased to 3.280ng/μL in mineralised dentine. The FEI-SEM analysis revealed positive immunolabelling patterns for MMP-3, predominantly localized on the intertubular collagen fibrillar network showing MMP-3 directly or indirectly bound to the collagen fibrils. Casein-zymograms showed positive proteolytic activity for MMP-3 in demineralised dentine powder.
conclusionThe results of the study clearly revealed the presence and distribution of MMP3 in human sound dentine. Whilst the presence was verified, its role is still unclear. Future studies are needed to investigate the possible involvement of MMP-3 in physiological and pathological condition of the dentine-pulp complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.