ArticleAnalytical biochemistry2011
Development and characterization of immobilized cannabinoid receptor (CB1/CB2) open tubular column for on-line screening.
Article in Analytical biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 47 citations in OpenAlex.
- The screened triterpene acids from Loquat fruit by CysLTR1-immobilized column could serve as alternative anti-inflammatory agents.BMC complementary medicine and therapies · 2025Article
- N-methyl-D-aspartic acid receptor 2A functionalized stationary phase: A reliable method for pursuing potential ligands against Alzheimer's disease from natural products.CNS neuroscience & therapeutics · 2023Article
- Preparation and characterization of immobilized 5-HTJournal of pharmaceutical and biomedical analysis · 2022Article
- Endocannabinoids in the Islets of Langerhans: the ugly, the bad, and the good facts.American journal of physiology. Endocrinology and metabolism · 2018Review
- Overview of the detection methods for equilibrium dissociation constantJournal of pharmaceutical analysis · 2018Review
- Comparison of analytical techniques for the identification of bioactive compounds from natural products.Natural product reports · 2016Review
- Human CB1 Receptor Isoforms, present in Hepatocytes and β-cells, are Involved in Regulating Metabolism.Scientific reports · 2016Article
- Development and characterization of the α3β4α5 nicotinic receptor cellular membrane affinity chromatography column and its application for on line screening of plant extracts.Journal of chromatography. A · 2016Article
- Targeting Anti-Cancer Active Compounds: Affinity-Based Chromatographic Assays.Current pharmaceutical design · 2016Review
- Highly stabilized, polymer-lipid membranes prepared on silica microparticles as stationary phases for capillary chromatography.Journal of chromatography. A · 2015Article
- In vivo inflammation imaging using a CB2R-targeted near infrared fluorescent probe.American journal of nuclear medicine and molecular imaging · 2015Article
- Multiple protein stationary phases: a review.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2014Review
- Comparative normal/failing rat myocardium cell membrane chromatographic analysis system for screening specific components that counteract doxorubicin-induced heart failure from Acontium carmichaeli.Analytical chemistry · 2014Article
- Identification of metabolites of propyrisulfuron in rats.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2014Article
- Stabilized phospholipid membranes in chromatography: toward membrane protein-functionalized stationary phases.Analytical and bioanalytical chemistry · 2014Article
- Identification of CB1/CB2 ligands from Zanthoxylum bungeanum.Journal of natural products · 2013Article
- Matching the power of high throughput screening to the chemical diversity of natural products.Natural product reports · 2013Review
- Development and preliminary validation of a plate-based CB1/CB2 receptor functional assay.Analytical biochemistry · 2013Article
- Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Cannabinoid receptors, CB1 and CB2, are therapeutic targets in the treatment of anxiety, obesity, movement disorders, glaucoma, and pain. We have developed an on-line screening method for CB1 and CB2 ligands, where cellular membrane fragments of a chronic myelogenous leukemia cell line, KU-812, were immobilized onto the surface of an open tubular (OT) capillary to create a CB1/CB2-OT column. The binding activities of the immobilized CB1/CB2 receptors were established using frontal affinity chromatographic techniques. This is the first report that confirms the presence of functional CB1 and CB2 receptors on KU-812 cells. The data from this study confirm that the CB1/CB2-OT column can be used to determine the binding affinities (K(i) values) for a single compound and to screen individual compounds or a mixture of multiple compounds. The CB1/CB2-OT column was also used to screen a botanical matrix, Zanthoxylum clava-herculis, where preliminary results suggest the presence of a high-affinity phytocannabinoid.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.