ArticleCell2011
Tunable signal processing in synthetic MAP kinase cascades.
Article in Cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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Who cites it
77 citing papers in PubMed.
- Protein engineering: status report.Protein engineering, design & selection : PEDS · 2026Review
- Kinase signaling cascades: an updated mechanistic landscape.Chemical science · 2025Review
- Sequence- and Docking-Site-Dependent Contributions to Multi-Site Phosphorylation of an Intrinsically Disordered MAPK Substrate.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Hierarchical metabolic engineering for rewiring cellular metabolism.FEMS microbiology reviews · 2025Review
- Nitrogen Deficiency Enhances Eggplant Defense against Western Flower Thrips via the Induction of the Jasmonate Pathway.Plants (Basel, Switzerland) · 2024Article
- Article
- A General Approach for the Modelling of Negative Feedback Physiological Control Systems.Bioengineering (Basel, Switzerland) · 2023Article
- Positive feedback induces switch between distributive and processive phosphorylation of Hog1.Nature communications · 2023Article
- Network regulation meets substrate modification chemistry.Journal of the Royal Society, Interface · 2023Article
- Article
- Symmetry breaking meets multisite modification.eLife · 2021Article
- Programmable protein circuit design.Cell · 2021Review
- Robust and flexible platform for directed evolution of yeast genetic switches.Nature communications · 2021Article
- Model-guided design of mammalian genetic programs.Science advances · 2021Article
- Investigating the Evolution and Development of Biological Systems from the Perspective of Thermo-Kinetics and Systems Theory.Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life · 2020Review
- Exploring cyclic networks of multisite modification reveals origins of information processing characteristics.Scientific reports · 2020Article
- Phosphoregulated orthogonal signal transduction in mammalian cells.Nature communications · 2020Article
- Synthetic metabolic computation in a bioluminescence-sensing system.Nucleic acids research · 2019Article
- Switching of INCENP paralogs controls transitions in mitotic chromosomal passenger complex functions.Cell cycle (Georgetown, Tex.) · 2019Article
- Development of efficient protocol for rice transformation overexpressing MAP kinase and their effect on root phenotypic traits.Protoplasma · 2019Article
17 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
The flexibility of MAPK cascade responses enables regulation of a vast array of cell fate decisions, but elucidating the mechanisms underlying this plasticity is difficult in endogenous signaling networks. We constructed insulated mammalian MAPK cascades in yeast to explore how intrinsic and extrinsic perturbations affect the flexibility of these synthetic signaling modules. Contrary to biphasic dependence on scaffold concentration, we observe monotonic decreases in signal strength as scaffold concentration increases. We find that augmenting the concentration of sequential kinases can enhance ultrasensitivity and lower the activation threshold. Further, integrating negative regulation and concentration variation can decouple ultrasensitivity and threshold from the strength of the response. Computational analyses show that cascading can generate ultrasensitivity and that natural cascades with different kinase concentrations are innately biased toward their distinct activation profiles. This work demonstrates that tunable signal processing is inherent to minimal MAPK modules and elucidates principles for rational design of synthetic signaling systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.