ArticleFree radical biology & medicine2011
Oxidative stress induces protein and DNA radical formation in follicular dendritic cells of the germinal center and modulates its cell death patterns in late sepsis.
Article in Free radical biology & medicine, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- PBX3 regulates mast cell parthanatos via TOP2A mediated DNA damage in allergic rhinitis.iScience · 2026Article
- Redox regulation of the immune response.Cellular & molecular immunology · 2022Review
- Follicular dendritic cell dysfunction contributes to impaired antigen-specific humoral responses in sepsis-surviving mice.The Journal of clinical investigation · 2021Article
- The impact of xanthine oxidase (XO) on hemolytic diseases.Redox biology · 2019Review
- Dendritic Cells in Sepsis: Pathological Alterations and Therapeutic Implications.Journal of immunology research · 2017Review
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- Tripping up Trp: Modification of protein tryptophan residues by reactive oxygen species, modes of detection, and biological consequences.Free radical biology & medicine · 2015Review
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- Phytochemical and in vitro biological investigations of methanolic extracts of Enhydra fluctuans Lour.Asian Pacific journal of tropical biomedicine · 2014Article
- Perturbed mononuclear phagocyte system in severely burned and septic patients.Shock (Augusta, Ga.) · 2013Review
- Proinflammatory adipokine leptin mediates disinfection byproduct bromodichloromethane-induced early steatohepatitic injury in obesity.Toxicology and applied pharmacology · 2013Article
- In vitro antioxidant, cytotoxic, thrombolytic activities and phytochemical evaluation of methanol extract of the A. philippense L. leaves.Asian Pacific journal of tropical biomedicine · 2013Article
- Leptin is key to peroxynitrite-mediated oxidative stress and Kupffer cell activation in experimental non-alcoholic steatohepatitis.Journal of hepatology · 2013Article
- Acetaminophen-induced acute liver injury in HCV transgenic mice.Toxicology and applied pharmacology · 2013Article
- An advanced Electron Spin Resonance (ESR) spin-trapping and LC/(ESR)/MS technique for the study of lipid peroxidation.International journal of molecular sciences · 2012Review
- In vivo imaging of immuno-spin trapped radicals with molecular magnetic resonance imaging in a diabetic mouse model.Diabetes · 2012Article
- Detection and imaging of the free radical DNA in cells--site-specific radical formation induced by Fenton chemistry and its repair in cellular DNA as seen by electron spin resonance, immuno-spin trapping and confocal microscopy.Nucleic acids research · 2012Article
- P2X7 receptor-NADPH oxidase axis mediates protein radical formation and Kupffer cell activation in carbon tetrachloride-mediated steatohepatitis in obese mice.Free radical biology & medicine · 2012Article
- Pseudolymphomatous luetic lymphadenitis associated with B cell clonality.Virchows Archiv : an international journal of pathology · 2012Article
- Xanthine oxidase inhibitors and sepsis.International journal of immunopathology and pharmacologyArticle
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Profound depletion of follicular dendritic cells (FDCs) is a hallmark of sepsis-like syndrome, but the exact causes of the ensuing cell death are unknown. The cell death-driven depletion contributes to immunoparalysis and is responsible for most of the morbidity and mortality in sepsis. Here we have utilized immuno-spin trapping, a method for detection of free radical formation, to detect oxidative stress-induced protein and DNA radical adducts in FDCs isolated from the spleens of septic mice and from human tonsil-derived HK cells, a subtype of germinal center FDCs, to study their role in FDC depletion. At 24h post-lipopolysaccharide administration, protein radical formation and oxidation were significantly elevated in vivo and in HK cells as shown by ELISA and confocal microscopy. The xanthine oxidase inhibitor allopurinol and the iron chelator desferrioxamine significantly decreased the formation of protein radicals, suggesting the role of xanthine oxidase and Fenton-like chemistry in radical formation. Protein and DNA radical formation correlated mostly with apoptotic features at 24h and necrotic morphology of all the cell types studied at 48h with concomitant inhibition of caspase-3. The cytotoxicity of FDCs resulted in decreased CD45R/CD138-positive plasma cell numbers, indicating a possible defect in B cell differentiation. In one such mechanism, radical formation initiated by xanthine oxidase formed protein and DNA radicals, which may lead to cell death of germinal center FDCs.
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