ArticleJournal of immunological methods2011
Image-based analysis of primary human neutrophil chemotaxis in an automated direct-viewing assay.
Article in Journal of immunological methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed.
- Pre-Activated Granulocytes from an Autoimmune Uveitis Model Show Divergent Pathway Activation Profiles upon IL8 Stimulation In Vitro.International journal of molecular sciences · 2022Article
- Gradient generation platforms: new directions for an established microfluidic technology.Lab on a chip · 2014Article
- Microfluidic transwell inserts for generation of tissue culture-friendly gradients in well plates.Lab on a chip · 2014Article
- Low-volume toolbox for the discovery of immunosuppressive fungal secondary metabolites.PLoS pathogens · 2013Article
- Microfluidic chambers for monitoring leukocyte trafficking and humanized nano-proresolving medicines interactions.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Quantitative analysis of gradient sensing: towards building predictive models of chemotaxis in cancer.Current opinion in cell biology · 2012Review
- On a chip.IEEE pulse · 2011Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Multi-well assays based on the Boyden chamber have enabled highly parallel studies of chemotaxis-the directional migration of cells in response to molecular gradients-while direct-viewing approaches have allowed more detailed questions to be asked at low throughput. Boyden-based plates provide a count of cells that pass through a membrane, but no information about cell appearance. In contrast, direct-viewing devices enable the observation of cells during chemotaxis, which allows measurement of many parameters including area, shape, and location. Here we show automated chemotaxis and cell morphology assays in a 96-unit direct-viewing plate. Using only 12000 primary human neutrophils per datum, we measured dose-dependent stimulation and inhibition of chemotaxis and quantified the effects of inhibitors on cell area and elongation. With 60 parallel conditions we demonstrated 5-fold increase in throughput compared to previously reported direct-viewing approaches.
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Registered trials
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