ArticleDevelopmental biology2011
FGF4-dependent stem cells derived from rat blastocysts differentiate along the trophoblast lineage.
Article in Developmental biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 67 citations in OpenAlex.
- Fundamental and unique roles of PLAC1 in the regulation of rat and human trophoblast cell development.Development (Cambridge, England) · 2026Article
- Directed differentiation of bovine trophoblast stem cells: A useful in vitro model for placenta development.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Leveraging chorionic villus biopsies for the derivation of patient-specific trophoblast stem cells.Communications biology · 2025Article
- Effects of an aryl hydrocarbon receptor ligand on human trophoblast cell development.Human reproduction (Oxford, England) · 2025Article
- Aryl Hydrocarbon Receptor Activation Drives 2-Methoxy Estradiol Secretion in Human Trophoblast Stem Cell Development.bioRxiv : the preprint server for biology · 2025Article
- Conserved and divergent features of trophoblast stem cells.Journal of molecular endocrinology · 2024Review
- Conditionally mutant animal model for investigating the invasive trophoblast cell lineage.Development (Cambridge, England) · 2024Article
- Evaluation of Molecular Interactions and Cellular Dynamics at the Maternal-Fetal Interface During Placental Morphogenesis.Methods in molecular biology (Clifton, N.J.) · 2024Article
- CITED2 is a conserved regulator of the uterine-placental interface.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The AKT1-FOXO4 axis reciprocally regulates hemochorial placentation.Development (Cambridge, England) · 2023Article
- Interleukin-15 deficient rats have reduced osteopontin at the maternal-fetal interface.Frontiers in cell and developmental biology · 2023Article
- Conservation at the uterine-placental interface.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Derivation of human triploid trophoblast stem cells.Journal of assisted reproduction and genetics · 2022Article
- Modeling Trophoblast Cell-Guided Uterine Spiral Artery Transformation in the Rat.International journal of molecular sciences · 2022Review
- Intersection of regulatory pathways controlling hemostasis and hemochorial placentation.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Evaluation of Placentation and the Role of the Aryl Hydrocarbon Receptor Pathway in a Rat Model of Dioxin Exposure.Environmental health perspectives · 2021Article
- Paradigms for investigating invasive trophoblast cell development and contributions to uterine spiral artery remodeling.Placenta · 2021Review
- SUV39H2 controls trophoblast stem cell fate.Biochimica et biophysica acta. General subjects · 2021Article
- ASCL2 reciprocally controls key trophoblast lineage decisions during hemochorial placenta development.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- SATB1 promotion of trophoblast stem cell renewal through regulation of threonine dehydrogenase.Biochimica et biophysica acta. General subjects · 2021Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Differentiated trophoblast cell lineages arise from trophoblast stem (TS) cells. To date such a stem cell population has only been established in the mouse. The objective of this investigation was to establish TS cell populations from rat blastocysts. Blastocysts were cultured individually on a feeder layer of rat embryonic fibroblasts (REFs) in fibroblast growth factor-4 (FGF4) and heparin supplemented culture medium. Once cell colonies were established REF feeder layers could be replaced with REF conditioned medium. The blastocyst-derived cell lines, in either proliferative or differentiated states, did not express genes indicative of ICM-derived tissues. In the proliferative state the cells expressed established stem cell-associated markers of TS cells. Cells ceased proliferation and differentiated when FGF4, heparin, and REF conditioned medium were removed. Differentiation was characterized by a decline of stem cell-associated marker gene expression, the appearance of large polyploid cells (trophoblast giant cells), and the expression of trophoblast differentiation-associated genes. Collectively, the data indicate that the rat blastocyst-derived cell lines not only possess many features characteristic of mouse TS cells but also possess some distinct properties. These rat TS cell lines represent valuable new in vitro models for analyses of mechanisms controlling TS cell renewal and differentiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.