ArticleImmunology2011
Identification of T-cell epitopes in Francisella tularensis using an ordered protein array of serological targets.
Article in Immunology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 25 citations in OpenAlex.
- Immunopeptidomics reveals conserved and abundant Francisella tularensis proteins as immunogenic CD4 T cell antigens in mice.PLoS pathogens · 2026Article
- Differential Immune Response Following Intranasal and Intradermal Infection withMicroorganisms · 2021Review
- Shotgun Immunoproteomic Approach for the Discovery of Linear B-Cell Epitopes in Biothreat AgentsFrontiers in immunology · 2021Article
- Adaptive Immunity toFrontiers in cellular and infection microbiology · 2018Review
- Epitopes of Immunoreactive Proteins ofFrontiers in cellular and infection microbiology · 2018Article
- Tularemia vaccines.Folia microbiologica · 2016Review
- Inclusion of Epitopes That Expand High-Avidity CD4+ T Cells Transforms Subprotective Vaccines to Efficacious Immunogens against Virulent Francisella tularensis.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Article
- Evolutionary Insights into IL17A in Lagomorphs.Mediators of inflammation · 2015Article
- T cell antigen discovery using soluble vaccinia proteome reveals recognition of antigens with both virion and nonvirion association.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- B-cell epitopes in GroEL of Francisella tularensis.PloS one · 2014Article
- CD4+ T cells provide intermolecular help to generate robust antibody responses in vaccinia virus-vaccinated humans.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Article
- Protective B-cell epitopes of Francisella tularensis O-polysaccharide in a mouse model of respiratory tularaemia.Immunology · 2012Article
- The utility and limitations of current Web-available algorithms to predict peptides recognized by CD4 T cells in response to pathogen infection.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Development of functional and molecular correlates of vaccine-induced protection for a model intracellular pathogen, F. tularensis LVS.PLoS pathogens · 2012Article
- Approaches to vaccines against Orientia tsutsugamushi.Frontiers in cellular and infection microbiology · 2012Review
- A mucosal subunit vaccine protects against lethal respiratory infection with Francisella tularensis LVS.PloS one · 2012Article
- A broadly applicable approach to T cell epitope identification: application to improving tumor associated epitopes and identifying epitopes in complex pathogens.Journal of immunological methods · 2011Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Francisella tularensis is a Gram-negative intracellular bacterium that is the causative agent of tularaemia. Concerns regarding its use as a bioterrorism agent have led to a renewed interest in the biology of infection, host response and pathogenesis. A robust T-cell response is critical to confer protection against F. tularensis. However, characterization of the cellular immune response has been hindered by the paucity of tools to examine the anti-Francisella immune response at the molecular level. We set out to combine recent advances of genomics with solid-phase antigen delivery coupled with a T-cell functional assay to identify T-cell epitopes. A subset of clones, encoding serological targets, was selected from an F. tularensis SchuS4 ordered genomic library and subcloned into a bacterial expression vector to test the feasibility of this approach. Proteins were expressed and purified individually employing the BioRobot 3000 in a semi-automated purification method. The purified proteins were coupled to beads, delivered to antigen-presenting cells for processing, and screened with Francisella-specific T-cell hybridomas of unknown specificity. We identified cellular reactivity against the pathogenicity protein IglB, and the chaperone proteins GroEL and DnaK. Further analyses using genetic deletions and synthetic peptides were performed to identify the minimal peptide epitopes. Priming with the peptide epitopes before infection with F. tularensis LVS increased the frequency of antigen-specific CD4 T cells as assessed by intracellular interferon-γ staining. These results illustrate the feasibility of screening an arrayed protein library that should be applicable to a variety of pathogens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.