ArticlePlant cell reports2011
Highly efficient Agrobacterium-mediated transformation of banana cv. Rasthali (AAB) via sonication and vacuum infiltration.
Article in Plant cell reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 98 citations in OpenAlex.
- Genetically Modified Plants in Agriculture.Biology · 2026Review
- Vacuum and Sonication Treatment Enable Efficient Transient Gene Expression in Various Monocot and Eudicot Plant Seedlings.ACS synthetic biology · 2026Article
- An EfficientPlants (Basel, Switzerland) · 2023Article
- Recalcitrance to transformation, a hindrance for genome editing of legumes.Frontiers in genome editing · 2023Review
- Biotechnology of banana (Musa spp.): multi-dimensional progress and prospect of in vitro-mediated system.Applied microbiology and biotechnology · 2022Review
- Purslane (Portulaca oleracea L.) as a novel green-bioreactor for expression of human serum albumin (HSA) gene.Transgenic research · 2022Article
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- Using Genetic Engineering Techniques to Develop Banana Cultivars With Fusarium Wilt Resistance and Ideal Plant Architecture.Frontiers in plant science · 2020Review
- A simple and fastPlant methods · 2020Article
- Efficient genetic transformation and CRISPR/Cas9-mediated genome editing in Lemna aequinoctialis.Plant biotechnology journal · 2019Article
- Genetic Transformation of Oat Mediated byIranian journal of biotechnology · 2019Article
- A simple and rapid protocol for the genetic transformation ofPlant methods · 2019Article
- Genotype-independent and enhanced in planta3 Biotech · 2018Article
- Improved Agrobacterium rhizogenes-mediated hairy root culture system of Withania somnifera (L.) Dunal using sonication and heat treatment.3 Biotech · 2015Article
- Agrobacterium-mediated in planta genetic transformation of sugarcane setts.Plant cell reports · 2015Article
- High efficiency transformation of banana [Musa acuminata L. cv. Matti (AA)] for enhanced tolerance to salt and drought stress through overexpression of a peanut salinity-induced pathogenesis-related class 10 protein.Molecular biotechnology · 2015Article
- Genetic transformation of date palm (Phoenix dactylifera L. cv. 'Estamaran') via particle bombardment.Molecular biology reports · 2014Article
- An efficient in planta transformation of Jatropha curcas (L.) and multiplication of transformed plants through in vivo grafting.Protoplasma · 2014Article
- Multiplex PCR assay for identification of commonly used disarmed Agrobacterium tumefaciens strains.SpringerPlus · 2014Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A reproducible and efficient transformation method was developed for the banana cv. Rasthali (AAB) via Agrobacterium-mediated genetic transformation of suckers. Three-month-old banana suckers were used as explant and three Agrobacterium tumefaciens strains (EHA105, EHA101, and LBA4404) harboring the binary vector pCAMBIA1301 were used in the co-cultivation. The banana suckers were sonicated and vacuum infiltered with each of the three A. tumefaciens strains and co-cultivated in the medium containing different concentrations of acetosyringone for 3 days. The transformed shoots were selected in 30 mg/l hygromycin-containing selection medium and rooted in rooting medium containing 1 mg/l IBA and 30 mg/l hygromycin. The presence and integration of the hpt II and gus genes into the banana genome were confirmed by GUS histochemical assay, polymerase chain reaction, and southern hybridization. Among the different combinations tested, high transformation efficiency (39.4 ± 0.5% GUS positive shoots) was obtained when suckers were sonicated and vacuum infiltered for 6 min with A. tumefaciens EHA105 in presence of 50 μM acetosyringone followed by co-cultivation in 50 μM acetosyringone-containing medium for 3 days. These results suggest that an efficient Agrobacterium-mediated transformation protocol for stable integration of foreign genes into banana has been developed and that this transformation system could be useful for future studies on transferring economically important genes into banana.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.