ArticleNature protocols2011
Isolation of circular yeast artificial chromosomes for synthetic biology and functional genomics studies.
Article in Nature protocols, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 51 citations in OpenAlex.
- Intergeneric chromosomal transfer in yeast results in improved phenotypes and widespread transcriptional responses.Nature communications · 2026Article
- Swine acute diarrhea syndrome coronavirus-related viruses from bats show potential interspecies infection.Journal of virology · 2025Article
- Development of viral infectious clones and their applications based on yeast and bacterial artificial chromosome platforms.Molecular biomedicine · 2025Review
- Efficient formation of single-copy human artificial chromosomes.Science (New York, N.Y.) · 2024Article
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- Reconstitution and Mutagenesis of Avian Infectious Laryngotracheitis Virus from Cosmid and Yeast Centromeric Plasmid Clones.Journal of virology · 2023Article
- Enzymatic Supercoiling of Bacterial Chromosomes Facilitates Genome Manipulation.ACS synthetic biology · 2022Article
- Highly Selective, CRISPR/Cas9-Mediated Isolation of Genes and Genomic Loci from Complex Genomes by TAR Cloning in Yeast.Current protocols · 2021Article
- Recent Advances in Strategies for the Cloning of Natural Product Biosynthetic Gene Clusters.Frontiers in bioengineering and biotechnology · 2021Review
- COMPASS for rapid combinatorial optimization of biochemical pathways based on artificial transcription factors.Nature communications · 2019Article
- Direct cloning and heterologous expression of natural product biosynthetic gene clusters by transformation-associated recombination.Methods in enzymology · 2019Article
- A Stable, Autonomously Replicating Plasmid Vector Containing Pichia pastoris Centromeric DNA.Applied and environmental microbiology · 2018Article
- Article
- AssemblX: a user-friendly toolkit for rapid and reliable multi-gene assemblies.Nucleic acids research · 2017Article
- Refined Pichia pastoris reference genome sequence.Journal of biotechnology · 2016Review
- Assembly of long DNA sequences using a new synthetic Escherichia coli-yeast shuttle vector.Virologica Sinica · 2016Article
- Highly efficient CRISPR/Cas9-mediated TAR cloning of genes and chromosomal loci from complex genomes in yeast.Nucleic acids research · 2015Article
- Genomics and Evolution in Traditional Medicinal Plants: Road to a Healthier Life.Evolutionary bioinformatics online · 2015Review
- Recombinase-mediated cassette exchange (RMCE) system for functional genomics studies in Mycoplasma mycoides.Biological procedures online · 2015Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
Circular yeast artificial chromosomes (YACs) provide significant advantages for cloning and manipulating large segments of genomic DNA in Saccharomyces cerevisiae. However, it has been difficult to exploit these advantages, because circular YACs are difficult to isolate and purify. Here we describe a method for purification of large circular YACs that is more reliable compared with previously described protocols. This method has been used to purify YACs up to 600 kb in size. The purified YAC DNA is suitable for restriction enzyme digestion, DNA sequencing and functional studies. For example, YACs carrying full-size genes can be purified from yeast and used for transfection into mammalian cells or for the construction of a synthetic genome that can be used to produce a synthetic cell. This method for isolating high-quality YAC DNA in microgram quantities should be valuable for functional and synthetic genomic studies. The entire protocol takes ∼3 d to complete.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.