ArticleNature protocols2011
Patch clamp recording from enteric neurons in situ.
Article in Nature protocols, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- Synaptic cell adhesion moleculeeLife · 2025Article
- Assessment of gastrointestinal function and enteric nervous system changes over time in the A53T mouse model of Parkinson's disease.Acta neuropathologica communications · 2025Article
- A novel method for culturing enteric neurons generates neurospheres containing functional myenteric neuronal subtypes.Journal of neuroscience methods · 2024Article
- Article
- The gut brain in a dish: Murine primary enteric nervous system cell cultures.Neurogastroenterology and motility · 2022Review
- Isolation and transfection of myenteric neurons from mice for patch-clamp applications.Frontiers in molecular neuroscience · 2022Article
- Opportunities and Challenges for Single-Unit Recordings from Enteric Neurons in Awake Animals.Micromachines · 2018Review
- Slow Wave Activity and Modulations in Mouse Jejunum Myenteric Plexus In Situ.Journal of neurogastroenterology and motility · 2017Article
- The Effect of Substrate Stiffness on Cardiomyocyte Action Potentials.Cell biochemistry and biophysics · 2016Article
- Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors.Journal of visualized experiments : JoVE · 2016Article
- Virtual leak channels modulate firing dynamics and synaptic integration in rat sympathetic neurons: implications for ganglionic transmission in vivo.The Journal of physiology · 2015Article
- Specialized functions of Nav1.5 and Nav1.9 channels in electrogenesis of myenteric neurons in intact mouse ganglia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Electrophysiological characteristics of enteric neurons isolated from the immortomouse.Digestive diseases and sciences · 2013Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The study of enteric neurons is key to understanding intestinal motility anGutn of therapeutic strategies for dealing with neurogenic disorders. However, enteric neurons have historically been inaccessible to patch-clamp recording. We report here the first technique that allows patch-clamp recording of neurons from the intact myenteric plexus of the mouse duodenum. The mucosa, submucosa and circular muscles are removed, exposing the myenteric plexus on the longitudinal muscle. Proteolytic treatment of exposed ganglia combined with gentle cell-surface cleaning allows gigaseal formation. Compared with previous studies using intracellular microelectrode recordings or cultured myenteric neurons, this technique provides an opportunity to explore properties of single or multiple ion channels in myenteric neurons in their native environment. The protocol-from the tissue preparation to patch-clamp recording-can be completed in ~4 h.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.