ArticleThe Journal of biological chemistry2011
The kinetic mechanism of mouse myosin VIIA.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- Tonotopic specialization of MYO7A isoforms in auditory hair cells.Nature communications · 2026Article
- Crosslinked F-actin networks regulate load-dependent energy conversion.Communications biology · 2026Article
- Mechanical power is maximized during contractile ring-like formation in a biomimetic dividing cell model.Nature communications · 2024Article
- F-actin architecture determines the conversion of chemical energy into mechanical work.Nature communications · 2024Article
- Myosin motors in sensory hair bundle assembly.Current opinion in cell biology · 2022Review
- Article
- Mutations in Drosophila crinkled/Myosin VIIA disrupt denticle morphogenesis.Developmental biology · 2021Article
- A new light chain for myosin-7.The Journal of biological chemistry · 2020Article
- The small EF-hand protein CALML4 functions as a critical myosin light chain within the intermicrovillar adhesion complex.The Journal of biological chemistry · 2020Article
- Single-molecule analysis reveals that regulatory light chains fine-tune skeletal myosin II function.The Journal of biological chemistry · 2020Article
- UsingDisease models & mechanisms · 2018Review
- The shaker-1 mouse myosin VIIa deafness mutation results in a severely reduced rate of the ATP hydrolysis step.The Journal of biological chemistry · 2018Article
- MyTH4-FERM myosins in the assembly and maintenance of actin-based protrusions.Current opinion in cell biology · 2017Review
- Kinetic Adaptations of Myosins for Their Diverse Cellular Functions.Traffic (Copenhagen, Denmark) · 2016Review
- Molecular characterization and subcellular localization of Arabidopsis class VIII myosin, ATM1.The Journal of biological chemistry · 2014Article
- Functional characterization of the human myosin-7a motor domain.Cellular and molecular life sciences : CMLS · 2012Article
- The ATPase mechanism of myosin 15, the molecular motor mutated in DFNB3 human deafness.The Journal of biological chemistryArticle
- Myosins in cell junctions.BioarchitectureReview
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
Abstract
Myosin VIIa is crucial in hearing and visual processes. We examined the kinetic and association properties of the baculovirus expressed, truncated mouse myosin VIIa construct containing the head, all 5IQ motifs and the putative coiled coil domain (myosin VIIa-5IQ). The construct appears to be monomeric as determined by analytical ultracentrifugation experiments, and only single headed molecules were detected by negative stain electron microscopy. The relatively high basal steady-state rate of 0.18 s(-1) is activated by actin only by ∼3.5-fold resulting in a V(max) of 0.7 s(-1) and a K(ATPase) of 11.5 μM. There is no single rate-limiting step of the ATP hydrolysis cycle. The ATP hydrolysis step (M·T M·D·P) is slow (12 s(-1)) and the equilibrium constant (K(H)) of 1 suggests significant reversal of hydrolysis. In the presence of actin ADP dissociates with a rate constant of 1.2 s(-1). Phosphate dissociation is relatively fast (>12 s(-1)), but the maximal rate could not be experimentally obtained at actin concentrations ≤ 50 μM because of the weak binding of the myosin VIIa-ADP-P(i) complex to actin. At higher actin concentrations the rate of attached hydrolysis (0.4 s(-1)) becomes significant and partially rate-limiting. Our findings suggest that the myosin VIIa is a "slow", monomeric molecular motor with a duty ratio of 0.6.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.