ArticleThe Journal of biological chemistry2011
A novel inhibitory domain of Helicobacter pylori protein CagA reduces CagA effects on host cell biology.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Article
- Gastric Epithelial Barrier Disruption, Inflammation and Oncogenic Signal Transduction by Helicobacter pylori.Current topics in microbiology and immunology · 2023Review
- Functional antagonism between CagA and DLC1 in gastric cancer.Cell death discovery · 2022Article
- Structural Analysis of Variability and Interaction of the N-terminal of the Oncogenic Effector CagA ofInternational journal of molecular sciences · 2018Article
- Variations in the multimerization region of theOncology letters · 2017Article
- Phospholipid binding residues of eukaryotic membrane-remodelling F-BAR domain proteins are conserved in Helicobacter pylori CagA.BMC research notes · 2014Article
- Helicobacter pylori in gastric carcinogenesis: mechanisms.Gastroenterology clinics of North America · 2013Review
- Helicobacter pylori cagA 12-bp insertion can be a marker for duodenal ulcer in Okinawa, Japan.Journal of gastroenterology and hepatology · 2013Article
- Article
- Caveolin-1 protects B6129 mice against Helicobacter pylori gastritis.PLoS pathogens · 2013Article
- Structural insights into Helicobacter pylori oncoprotein CagA interaction with β1 integrin.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Conserved transcriptional unit organization of the cag pathogenicity island among Helicobacter pylori strains.Frontiers in cellular and infection microbiology · 2012Article
- "Targeted disruption of the epithelial-barrier by Helicobacter pylori".Cell communication and signaling : CCS · 2011Article
- Attenuated CagA oncoprotein in Helicobacter pylori from Amerindians in Peruvian Amazon.The Journal of biological chemistry · 2011Article
- Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Helicobacter pylori protein CagA (cytotoxin-associated gene A) is associated with an increased risk for gastric cancer formation. After attachment to epithelial cells, the bacteria inject CagA via a type IV secretion apparatus into host cells, where it exerts its biological activity. Host cell responses to intracellular CagA have been linked exclusively to signaling motifs in the C terminus of the CagA protein. Little is known about the functional role of the remaining CagA protein. Using transgenic expression of CagA mutants in epithelial cells, we were able to identify a novel CagA inhibitory domain at the N terminus consisting of the first 200 amino acids. This domain localizes to cell-cell contacts and increases the rate and strength of cell-cell adhesion in epithelial cells. Thus, it compensates for the loss of cell-cell adhesion induced by the C terminus of the CagA protein. Consistent with its stabilizing role on cell-cell adhesion, the CagA N terminus domain reduces the CagA-induced β-catenin transcriptional activity in the nucleus. Furthermore, it inhibits apical surface constriction and cell elongations, host cell phenotypes induced by the C terminus in polarized epithelia. Therefore, our study suggests that CagA contains an intrinsic inhibitory domain that reduces host cell responses to CagA, which have been associated with the formation of cancer.
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