ArticleMicrobiology (Reading, England)2011
Ler of pathogenic Escherichia coli forms toroidal protein-DNA complexes.
Article in Microbiology (Reading, England), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
- Structural basis of the oligomerization of anti-silencer Ler from enterohemorrhagicStructural dynamics (Melville, N.Y.) · 2026Article
- Article
- Review
- High Frequency of Multidrug-Resistant (MDR) Atypical EnteropathogenicFrontiers in veterinary science · 2020Article
- Impact of Xenogeneic Silencing on Phage-Host Interactions.Journal of molecular biology · 2019Review
- Article
- Environment Controls LEE Regulation in EnteropathogenicFrontiers in microbiology · 2018Review
- Regulation ofEcoSal Plus · 2017Review
- H-NS Regulates Gene Expression and Compacts the Nucleoid: Insights from Single-Molecule Experiments.Biophysical journal · 2015Review
- Integrated circuits: how transcriptional silencing and counter-silencing facilitate bacterial evolution.Current opinion in microbiology · 2015Review
- Silencing by H-NS potentiated the evolution of Salmonella.PLoS pathogens · 2014Article
- DNA looping-dependent autorepression of LEE1 P1 promoters by Ler in enteropathogenic Escherichia coli (EPEC).Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Locus of enterocyte effacement-encoded regulator (Ler) of pathogenic Escherichia coli competes off histone-like nucleoid-structuring protein (H-NS) through noncooperative DNA binding.The Journal of biological chemistry · 2014Article
- H-NST induces LEE expression and the formation of attaching and effacing lesions in enterohemorrhagic Escherichia coli.PloS one · 2014Article
- Microarray analysis of the Ler regulon in enteropathogenic and enterohaemorrhagic Escherichia coli strains.PloS one · 2014Article
- Oligomerization and DNA binding of Ler, a master regulator of pathogenicity of enterohemorrhagic and enteropathogenic Escherichia coli.Nucleic acids research · 2012Article
- A distinct regulatory sequence is essential for the expression of a subset of nle genes in attaching and effacing Escherichia coli.Journal of bacteriology · 2012Article
- Zinc-induced envelope stress diminishes type III secretion in enteropathogenic Escherichia coli.BMC microbiology · 2012Article
- The 5.5 protein of phage T7 inhibits H-NS through interactions with the central oligomerization domain.Journal of bacteriology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 5 institutions in 1 country.
Funding
Abstract
Enteropathogenic and enterohaemorrhagic Escherichia coli are related pathotypes of bacteria that cause acute watery diarrhoea and haemorrhagic colitis, respectively, and enterohaemorrhagic E. coli can lead to a serious complication known as haemolytic uraemic syndrome. In both bacteria the global regulatory protein Ler controls virulence. The ler gene is found within the locus of enterocyte effacement, or LEE, encoding a type III secretion system necessary for injecting effector proteins into intestinal epithelial cells and causing net secretory diarrhoea. The nucleoid-associated protein H-NS silences, whereas Ler serves as an anti-silencer of, multiple LEE operons. Although Ler has a higher affinity for DNA than does H-NS, the precise molecular mechanism by which Ler increases LEE transcription remains to be determined. In this report we investigate the oligomerization activity of Ler. In solution, Ler forms dimers and soluble aggregates of up to 5000 kDa molecular mass, and appears to oligomerize more readily than the related protein H-NS. An insertional mutation into the Ler linker region diminished oligomerization activity. Despite being proteins of similar mass and having homologous DNA-binding domains, Ler and H-NS complexed to DNA migrated to distinct locations, as determined by an electrophoretic mobility shift assay, implying that the related proteins form different 3D shapes in the presence of DNA. Lastly, we present electron microscopy images of toroidal Ler-DNA structures that are predicted to be involved in stimulating gene expression.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.