ArticleJournal of fluorescence2011
Quantitative analysis of intracellular calcium and mitochondrial kinetic fluorescence changes in GSNO-induced thymocyte early apoptosis.
Article in Journal of fluorescence, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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0 citing papers in PubMed, 2 citations in OpenAlex.
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3 authors at 2 institutions in 1 country.
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Abstract
A fluorescence microscopy imaging technique was applied to observe the single-cell kinetic changes of intracellular Ca(2+) concentration ([Ca(2+)](i)) and mitochondrial membrane potential (ΔΨ(m)) during the early stage of S-nitrosoglutathione (GSNO)-induced thymocytes apoptosis. The kinetic features of [Ca(2+)](i) and ΔΨ(m) were quantitatively analyzed and compared by fitting the fluorescence intensity data. The mathematical parameter, inflection point which indicated the time point when [Ca(2+)](i) or ΔΨ(m) changed the most rapidly, was proposed to analyze the fitting curve. The results revealed that the inflection point of [Ca(2+)](i) always appeared prior to that of ΔΨ(m) during apoptosis induced by a certain GSNO concentration. Both the [Ca(2+)](i) and ΔΨ(m) changed in a GSNO concentration-dependent manner. Another parameter, half-max effect point was also employed and displayed the similar results. Such quantitative analyses of real-time observations at the single-cell level are useful for interpreting the sequence of the biological events operating in GSNO-induced thymocyte apoptosis.
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