ArticleThe Journal of investigative dermatology2011
The calcium-sensing receptor-dependent regulation of cell-cell adhesion and keratinocyte differentiation requires Rho and filamin A.
Article in The Journal of investigative dermatology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 59 citations in OpenAlex.
- Method and Analysis of High-Calcium Induced Differentiation of Human Primary KeratinocytesMethods and protocols · 2026Article
- Various Cellular Components and Its Signaling Cascades Through the Involvement of Signaling Messengers in Keratinocyte Differentiation.Antioxidants (Basel, Switzerland) · 2025Review
- Dysregulation of STS in keratinocytes promotes calcium signaling and differentiation.Scientific reports · 2025Article
- Keratinocyte-derived VEGF-A is an essential pro-migratory autocrine mediator, acting through the KDR/GEF-H1/RhoA pathway.Frontiers in cell and developmental biology · 2025Article
- CaCellular signalling · 2023Review
- Mechanotransduction in Skin Inflammation.Cells · 2022Review
- Decreased Calcium-Sensing Receptor Expression Controls Calcium Signaling and Cell-To-Cell Adhesion Defects in Aged Skin.The Journal of investigative dermatology · 2021Article
- Caffeoyl-Prolyl-Histidine Amide Inhibits Fyn and Alleviates Atopic Dermatitis-Like Phenotypes via Suppression of NF-κB Activation.International journal of molecular sciences · 2020Article
- Calcium-sensing receptor deletion in the mouse esophagus alters barrier function.American journal of physiology. Gastrointestinal and liver physiology · 2020Article
- Fucoidan fromMarine drugs · 2019Article
- Calcium-Sensing Receptor Regulates Epidermal Intracellular CaThe Journal of investigative dermatology · 2019Article
- Establishment of a complex skin structure via layered co-culture of keratinocytes and fibroblasts derived from induced pluripotent stem cells.Stem cell research & therapy · 2018Article
- Scaffolding proteins in the development and maintenance of the epidermal permeability barrier.Tissue barriers · 2017Review
- Signaling regulation and role of filamin A cleavage in Ca2+-stimulated migration of androgen receptor-deficient prostate cancer cells.Oncotarget · 2017Article
- Disruption of Vitamin D and Calcium Signaling in Keratinocytes Predisposes to Skin Cancer.Frontiers in physiology · 2016Review
- An updated review of mechanotransduction in skin disorders: transcriptional regulators, ion channels, and microRNAs.Cellular and molecular life sciences : CMLS · 2015Review
- Vitamin D receptor, a tumor suppressor in skin.Canadian journal of physiology and pharmacology · 2015Review
- Novel mechanisms for the vitamin D receptor (VDR) in the skin and in skin cancer.The Journal of steroid biochemistry and molecular biology · 2015Review
- Obligatory roles of filamin A in E-cadherin-mediated cell-cell adhesion in epidermal keratinocytes.Journal of dermatological science · 2014Article
- Recombinant Treponema pallidum protein Tp0965 activates endothelial cells and increases the permeability of endothelial cell monolayer.PloS one · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Extracellular Ca(2+) (Ca(2+)(o)) functioning through the calcium-sensing receptor (CaR) induces E-cadherin-mediated cell-cell adhesion and cellular signals mediating cell differentiation in epidermal keratinocytes. Previous studies indicate that CaR regulates cell-cell adhesion through Fyn/Src tyrosine kinases. In this study, we investigate whether Rho GTPase is a part of the CaR-mediated signaling cascade regulating cell adhesion and differentiation. Suppressing endogenous Rho A expression by small interfering RNA (siRNA)-mediated gene silencing blocked the Ca(2+)(o)-induced association of Fyn with E-cadherin and suppressed the Ca(2+)(o)-induced tyrosine phosphorylation of β-, γ-, and p120-catenin and formation of intercellular adherens junctions. Rho A silencing also decreased the Ca(2+)(o)-stimulated expression of terminal differentiation markers. Elevating the Ca(2+)(o) level induced interactions among CaR, Rho A, E-cadherin, and the scaffolding protein filamin A at the cell membrane. Inactivation of CaR expression by adenoviral expression of a CaR antisense complementary DNA inhibited Ca(2+)(o)-induced activation of endogenous Rho. Ca(2+)(o) activation of Rho required a direct interaction between CaR and filamin A. Interference of CaR-filamin interaction inhibited Ca(2+)(o)-induced Rho activation and the formation of cell-cell junctions. These results indicate that Rho is a downstream mediator of CaR in the regulation of Ca(2+)(o)-induced E-cadherin-mediated cell-cell adhesion and keratinocyte differentiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.