ArticleBiology of reproduction2011
Suppression of Stra8 expression in the mouse gonad by WIN 18,446.
Article in Biology of reproduction, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 65 citations in OpenAlex.
- Reviewing the Implication of Aldehyde Dehydrogenases in Male Reproduction: Prospects for New Therapeutic Approaches.Pharmaceuticals (Basel, Switzerland) · 2026Review
- Programming for Meiotic Competence in Mouse Male Germ Cells is Established at the Perinatal Precursor Stage of Development.Molecular reproduction and development · 2025Article
- Inhibiting Aldehyde Dehydrogenase Function Using WIN 18,446 to Synchronize Spermatogenesis.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Temporal maturation of Sertoli cells during the establishment of the cycle of the seminiferous epithelium†.Biology of reproduction · 2024Article
- Review
- Modeling mammalian spermatogonial differentiation and meiotic initiation in vitro.Development (Cambridge, England) · 2022Article
- Synchronizing spermatogenesis in the mouse.Biology of reproduction · 2022Article
- How does retinoic acid (RA) signaling pathway regulate spermatogenesis?Histology and histopathology · 2022Review
- Review
- Two distinct Sertoli cell states are regulated via germ cell crosstalk†.Biology of reproduction · 2021Article
- Mammalian SWI/SNF chromatin remodeler is essential for reductional meiosis in males.Nature communications · 2021Article
- Transient suppression of transplanted spermatogonial stem cell differentiation restores fertility in mice.Cell stem cell · 2021Article
- STRA8 induces transcriptional changes in germ cells during spermatogonial development.Molecular reproduction and development · 2021Article
- Reduced retinoic acid synthesis accelerates prophase I and follicle activation.Reproduction (Cambridge, England) · 2020Article
- Review
- Retinoic Acid and Germ Cell Development in the Ovary and Testis.Biomolecules · 2019Review
- Retinoic acid signaling and the cycle of the seminiferous epithelium.Current opinion in endocrine and metabolic research · 2019Article
- Sources of all-trans retinal oxidation independent of the aldehyde dehydrogenase 1A isozymes exist in the postnatal testis†.Biology of reproduction · 2019Article
- 50 years of spermatogenesis: Sertoli cells and their interactions with germ cells.Biology of reproduction · 2018Review
- Beyond stem cells: Commitment of progenitor cells to meiosis.Stem cell research · 2018Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Bis-(dichloroacetyl)-diamines (BDADs) are compounds that inhibit spermatogenesis and function as male contraceptives in many species; however, their mechanism of action has yet to be fully investigated. It has been proposed that BDADs may function via inhibition of testicular retinoic acid (RA) biosynthesis. We employed an organ culture technique and the expression of a marker for RA activity, Stra8 (stimulated by retinoic acid gene 8), to investigate if the BDAD WIN 18,446 inhibited the biosynthesis of RA from retinol (ROL) in neonatal and adult murine testis and in the embryonic murine gonad. After culturing either whole testes or germ cells isolated from mice at 2 days postpartum (dpp) with WIN 18,446 or with WIN 18,446 plus ROL, Stra8 expression was suppressed, demonstrating that WIN 18,446 inhibited the conversion of ROL to RA in both systems. We also utilized a transgenic mouse containing an RA-responsive LacZ reporter gene to demonstrate limited RA induction of LacZ expression in 2-dpp testes cultured with WIN 18,446 plus ROL. The expression of Stra8 was downregulated in adult mouse testis tubules cultured with WIN 18,446 when compared to tubules cultured with the vehicle control. WIN 18,446 also inhibited the conversion of ROL to RA in embryonic ovaries and testes cultured for 48 h. These murine results provide critical insights regarding how the BDADs can inhibit spermatogenesis by blocking the ability of vitamin A to drive germ cell development. In addition, these techniques will be useful for screening novel inhibitors of RA biosynthesis as potential male contraceptives.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.