ArticleMolecular biology of the cell2011
Exo-endocytic trafficking and the septin-based diffusion barrier are required for the maintenance of Cdc42p polarization during budding yeast asymmetric growth.
Article in Molecular biology of the cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Septin Organization and Dynamics for Budding Yeast Cytokinesis.Journal of fungi (Basel, Switzerland) · 2024Review
- Red CdSe/ZnS QDs' Intracellular Trafficking and Its Impact on Yeast Polarization and Actin Filament.Cells · 2023Article
- Essential role of the endocytic site-associated protein Ecm25 in stress-induced cell elongation.Cell reports · 2021Article
- Review
- The Cdc42 effectors Gic1 and Gic2 regulate polarized post-Golgi secretion.Cell & bioscience · 2019Article
- Differential GAP requirement for Cdc42-GTP polarization during proliferation and sexual reproduction.The Journal of cell biology · 2018Article
- Phosphatidylserine and GTPase activation control Cdc42 nanoclustering to counter dissipative diffusion.Molecular biology of the cell · 2018Article
- Role of Arf GTPases in fungal morphogenesis and virulence.PLoS pathogens · 2017Article
- Remodeling of the Fission Yeast Cdc42 Cell-Polarity Module via the Sty1 p38 Stress-Activated Protein Kinase Pathway.Current biology : CB · 2016Article
- Polarized Exocytosis Induces Compensatory Endocytosis by Sec4p-Regulated Cortical Actin Polymerization.PLoS biology · 2016Article
- Septin Organization and Functions in Budding Yeast.Frontiers in cell and developmental biology · 2016Review
- Spontaneous Cdc42 polarization independent of GDI-mediated extraction and actin-based trafficking.PLoS biology · 2015Article
- Septins and Generation of Asymmetries in Fungal Cells.Annual review of microbiology · 2015Review
- Quantitative analysis of membrane trafficking in regulation of Cdc42 polarity.Traffic (Copenhagen, Denmark) · 2014Article
- Sequential logic of polarity determination during the haploid-to-diploid transition in Saccharomyces cerevisiae.Eukaryotic cell · 2014Article
- Article
- Bem3: Filling the GAP between cell polarity and secretion.Communicative & integrative biology · 2013Article
- Bem3, a Cdc42 GTPase-activating protein, traffics to an intracellular compartment and recruits the secretory Rab GTPase Sec4 to endomembranes.Journal of cell science · 2013Article
- Robust polarity establishment occurs via an endocytosis-based cortical corralling mechanism.The Journal of cell biology · 2013Article
- Non-uniform membrane diffusion enables steady-state cell polarization via vesicular trafficking.Nature communications · 2013Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Cdc42p plays a central role in asymmetric cell growth in yeast by controlling actin organization and vesicular trafficking. However, how Cdc42p is maintained specifically at the daughter cell plasma membrane during asymmetric cell growth is unclear. We have analyzed Cdc42p localization in yeast mutants defective in various stages of membrane trafficking by fluorescence microscopy and biochemical fractionation. We found that two separate exocytic pathways mediate Cdc42p delivery to the daughter cell. Defects in one of these pathways result in Cdc42p being rerouted through the other. In particular, the pathway involving trafficking through endosomes may couple Cdc42p endocytosis from, and subsequent redelivery to, the plasma membrane to maintain Cdc42p polarization at the daughter cell. Although the endo-exocytotic coupling is necessary for Cdc42p polarization, it is not sufficient to prevent the lateral diffusion of Cdc42p along the cell cortex. A barrier function conferred by septins is required to counteract the dispersal of Cdc42p and maintain its localization in the daughter cell but has no effect on the initial polarization of Cdc42p at the presumptive budding site before symmetry breaking. Collectively, membrane trafficking and septins function synergistically to maintain the dynamic polarization of Cdc42p during asymmetric growth in yeast.
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