ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2011
Depolarizing actions of GABA in immature neurons depend neither on ketone bodies nor on pyruvate.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 59 citations in OpenAlex.
- Step by step: cells with multiple functions in cortical circuit assembly.Nature reviews. Neuroscience · 2022Review
- The Paroxysmal Depolarization Shift: Reconsidering Its Role in Epilepsy, Epileptogenesis and Beyond.International journal of molecular sciences · 2019Review
- Review
- Endogenous GABA controls oligodendrocyte lineage cell number, myelination, and CNS internode length.Glia · 2017Article
- An Optogenetic Approach for Investigation of Excitatory and Inhibitory Network GABA Actions in Mice Expressing Channelrhodopsin-2 in GABAergic Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- GABA depolarizes immature neurons and inhibits network activity in the neonatal neocortex in vivo.Nature communications · 2015Article
- Neuron-astrocyte interaction enhance GABAergic synaptic transmission in a manner dependent on key metabolic enzymes.Frontiers in cellular neuroscience · 2015Article
- Genetic and pharmacological modulation of giant depolarizing potentials in the neonatal hippocampus associates with increased seizure susceptibility.The Journal of physiology · 2013Article
- Ketone bodies in epilepsy.Journal of neurochemistry · 2012Review
- Traumatic alterations in GABA signaling disrupt hippocampal network activity in the developing brain.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Synaptic activity-induced Ca(2+) signaling in avian cochlear nucleus magnocellularis neurons.Neuroscience research · 2012Article
- Article
- Role of the neuronal K-Cl co-transporter KCC2 in inhibitory and excitatory neurotransmission.Frontiers in cellular neuroscience · 2012Article
- Excitatory GABA: How a Correct Observation May Turn Out to be an Experimental Artifact.Frontiers in pharmacology · 2012Article
- GABA metabolism and transport: effects on synaptic efficacy.Neural plasticity · 2012Review
- Refuting the challenges of the developmental shift of polarity of GABA actions: GABA more exciting than ever!Frontiers in cellular neuroscience · 2012Article
- Is it safe to use a diuretic to treat seizures early in development ?Epilepsy currents · 2011Article
- Emerging themes in GABAergic synapse development.Progress in neurobiology · 2011Review
- Chronic hyperosmotic stress converts GABAergic inhibition into excitation in vasopressin and oxytocin neurons in the rat.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Efficacy of synaptic inhibition depends on multiple, dynamically interacting mechanisms implicated in chloride homeostasis.PLoS computational biology · 2011Article
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Authors and funding
19 authors at 8 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
GABA depolarizes immature neurons because of a high [Cl(-)](i) and orchestrates giant depolarizing potential (GDP) generation. Zilberter and coworkers (Rheims et al., 2009; Holmgren et al., 2010) showed recently that the ketone body metabolite DL-3-hydroxybutyrate (DL-BHB) (4 mM), lactate (4 mM), or pyruvate (5 mM) shifted GABA actions to hyperpolarizing, suggesting that the depolarizing effects of GABA are attributable to inadequate energy supply when glucose is the sole energy source. We now report that, in rat pups (postnatal days 4-7), plasma D-BHB, lactate, and pyruvate levels are 0.9, 1.5, and 0.12 mM, respectively. Then, we show that DL-BHB (4 mM) and pyruvate (200 μM) do not affect (i) the driving force for GABA(A) receptor-mediated currents (DF(GABA)) in cell-attached single-channel recordings, (2) the resting membrane potential and reversal potential of synaptic GABA(A) receptor-mediated responses in perforated patch recordings, (3) the action potentials triggered by focal GABA applications, or (4) the GDPs determined with electrophysiological recordings and dynamic two-photon calcium imaging. Only very high nonphysiological concentrations of pyruvate (5 mM) reduced DF(GABA) and blocked GDPs. Therefore, DL-BHB does not alter GABA signals even at the high concentrations used by Zilberter and colleagues, whereas pyruvate requires exceedingly high nonphysiological concentrations to exert an effect. There is no need to alter conventional glucose enriched artificial CSF to investigate GABA signals in the developing brain.
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