ArticleUltrasound in medicine & biology2011
Gene expression analysis of mouse embryonic stem cells following levitation in an ultrasound standing wave trap.
Article in Ultrasound in medicine & biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed, 56 citations in OpenAlex.
- Sound innovations for biofabrication and tissue engineering.Microsystems & nanoengineering · 2024Review
- Ultrasound-induced reorientation for multi-angle optical coherence tomography.Nature communications · 2024Article
- Monitoring Live Mycobacteria in Real-Time Using a Microfluidic Acoustic-Raman Platform.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Real-time monitoring of live mycobacteria with a microfluidic acoustic-Raman platform.Communications biology · 2020Article
- Engineering multi-layered tissue constructs using acoustic levitation.Scientific reports · 2019Article
- Light sheet microscopy with acoustic sample confinement.Nature communications · 2019Article
- Generation of functional hepatocyte 3D discoids in an acoustofluidic bioreactor.Biomicrofluidics · 2019Article
- Ultrasonic Based Tissue Modelling and Engineering.Micromachines · 2018Review
- Characterizing Deformability of Drug Resistant Patient-Derived Acute Lymphoblastic Leukemia (ALL) Cells Using Acoustic Tweezers.Scientific reports · 2018Article
- Formation of cell spheroids using Standing Surface Acoustic Wave (SSAW).International journal of bioprinting · 2018Article
- Rapid formation of size-controllable multicellular spheroids via 3D acoustic tweezers.Lab on a chip · 2016Article
- Effects of acoustic levitation on the development of zebrafish, Danio rerio, embryos.Scientific reports · 2015Article
- Deformation of red blood cells using acoustic radiation forces.Biomicrofluidics · 2014Article
- Microchannel acoustophoresis does not impact survival or function of microglia, leukocytes or tumor cells.PloS one · 2013Article
- Combined acoustic and optical trapping.Biomedical optics express · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
In the present paper, gene expression analysis of mouse embryonic stem (ES) cells levitated in a novel ultrasound standing wave trap (USWT) (Bazou et al. 2005a) at variable acoustic pressures (0.08-0.85 MPa) and times (5-60 min) was performed. Our results showed that levitation of ES cells at the highest employed acoustic pressure for 60 min does not modify gene expression and cells maintain their pluripotency. Embryoid bodies (EBs) also expressed the early and late neural differentiation markers, which were also unaffected by the acoustic field. Our results suggest that the ultrasound trap microenvironment is minimally invasive as the biologic consequences of ES cell replication and EB differentiation proceed without significantly affecting gene expression. The technique holds great promise in safe cell manipulation techniques for a variety of applications including tissue engineering and regenerative medicine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.