ArticlePloS one2010
Approaching the secrets of N-glycosylation in Aspergillus fumigatus: characterization of the AfOch1 protein.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 42 citations in OpenAlex.
- Integrative perspectives on glycosylation networks in fungi and oomycetes.Journal of microbiology (Seoul, Korea) · 2025Review
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- Role of sphingolipids in the host-pathogen interaction.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2023Article
- Role of Protein Glycosylation in Interactions of Medically Relevant Fungi with the Host.Journal of fungi (Basel, Switzerland) · 2021Review
- Post-Translational Modifications Drive Success and Failure of Fungal-Host Interactions.Journal of fungi (Basel, Switzerland) · 2021Review
- Galactomannan Produced byJournal of fungi (Basel, Switzerland) · 2020Review
- CoremBio · 2020Article
- Silencing ofInfection and drug resistance · 2019Article
- Pathogenesis of Dermatophytosis: Sensing the Host Tissue.Mycopathologia · 2017Review
- Recent advances in the understanding of the Aspergillus fumigatus cell wall.Journal of microbiology (Seoul, Korea) · 2016Review
- More Than Just Oligomannose: An N-glycomic Comparison of Penicillium Species.Molecular & cellular proteomics : MCP · 2016Article
- Co-ordination between BrlA regulation and secretion of the oxidoreductase FmqD directs selective accumulation of fumiquinazoline C to conidial tissues in Aspergillus fumigatus.Cellular microbiology · 2014Article
- Cryptococcus neoformans dual GDP-mannose transporters and their role in biology and virulence.Eukaryotic cell · 2014Article
- The Fusarium oxysporum gnt2, encoding a putative N-acetylglucosamine transferase, is involved in cell wall architecture and virulence.PloS one · 2013Article
- Biosynthesis of the fungal cell wall polysaccharide galactomannan requires intraluminal GDP-mannose.The Journal of biological chemistry · 2012Article
- Unraveling unique structure and biosynthesis pathway of N-linked glycans in human fungal pathogen Cryptococcus neoformans by glycomics analysis.The Journal of biological chemistry · 2012Article
- Transcription of N- and O-linked mannosyltransferase genes is modulated by the pacC gene in the human dermatophyte Trichophyton rubrum.FEBS open bio · 2012Article
- Protein Glycosylation in Aspergillus fumigatus Is Essential for Cell Wall Synthesis and Serves as a Promising Model of Multicellular Eukaryotic Development.International journal of microbiology · 2012Article
- The two-component sensor kinase TcsC and its role in stress resistance of the human-pathogenic mold Aspergillus fumigatus.PloS one · 2012Article
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mannosyltransferase Och1 is the key enzyme for synthesis of elaborated protein N-glycans in yeast. In filamentous fungi genes implicated in outer chain formation are present, but their function is unclear. In this study we have analyzed the Och1 protein of Aspergillus fumigatus. We provide first evidence that poly-mannosylated N-glycans exist in A. fumigatus and that their synthesis requires AfOch1 activity. This implies that AfOch1 plays a similar role as S. cerevisiae ScOch1 in the initiation of an N-glycan outer chain. A Δafoch1 mutant showed normal growth under standard and various stress conditions including elevated temperature, cell wall and oxidative stress. However, sporulation of this mutant was dramatically reduced in the presence of high calcium concentrations, suggesting that certain proteins engaged in sporulation require N-glycan outer chains to be fully functional. A characteristic feature of AfOch1 and Och1 homologues from other filamentous fungi is a signal peptide that clearly distinguishes them from their yeast counterparts. However, this difference does not appear to have consequences for its localization in the Golgi. Replacing the signal peptide of AfOch1 by a membrane anchor had no impact on its ability to complement the sporulation defect of the Δafoch1 strain. The mutant triggered a normal cytokine response in infected murine macrophages, arguing against a role of outer chains as relevant Aspergillus pathogen associated molecular patterns. Infection experiments provided no evidence for attenuation in virulence; in fact, according to our data the Δafoch1 mutant may even be slightly more virulent than the control strains.
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