ArticlePloS one2010
A systematic enhancer screen using lentivector transgenesis identifies conserved and non-conserved functional elements at the Olig1 and Olig2 locus.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Article
- Identifying an oligodendrocyte enhancer that regulates Olig2 expression.Human molecular genetics · 2023Article
- Heart Enhancers: Development and Disease Control at a Distance.Frontiers in genetics · 2021Review
- Similarities and differences in the regulation of HoxD genes during chick and mouse limb development.PLoS biology · 2018Article
- Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders.Journal of visualized experiments : JoVE · 2018Article
- Control of Hoxd gene transcription in the mammary bud by hijacking a preexisting regulatory landscape.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Functionally conserved enhancers with divergent sequences in distant vertebrates.BMC genomics · 2015Article
- A discrete transition zone organizes the topological and regulatory autonomy of the adjacent tfap2c and bmp7 genes.PLoS genetics · 2015Article
- Identification of a transient Sox5 expressing progenitor population in the neonatal ventral forebrain by a novel cis-regulatory element.Developmental biology · 2014Article
- Article
- Sox10-MCS5 enhancer dynamically tracks human oligodendrocyte progenitor fate.Experimental neurology · 2013Article
- A yeast one-hybrid and microfluidics-based pipeline to map mammalian gene regulatory networks.Molecular systems biology · 2013Article
- Stage-specific deletion of Olig2 conveys opposing functions on differentiation and maturation of oligodendrocytes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- From remote enhancers to gene regulation: charting the genome's regulatory landscapes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- SOX2 co-occupies distal enhancer elements with distinct POU factors in ESCs and NPCs to specify cell state.PLoS genetics · 2013Article
- Shadow enhancers: frequently asked questions about distributed cis-regulatory information and enhancer redundancy.BioEssays : news and reviews in molecular, cellular and developmental biology · 2012Article
- Engineering the embryo.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Chromosome conformation capture uncovers potential genome-wide interactions between human conserved non-coding sequences.PloS one · 2011Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Finding sequences that control expression of genes is central to understanding genome function. Previous studies have used evolutionary conservation as an indicator of regulatory potential. Here, we present a method for the unbiased in vivo screen of putative enhancers in large DNA regions, using the mouse as a model. We cloned a library of 142 overlapping fragments from a 200 kb-long murine BAC in a lentiviral vector expressing LacZ from a minimal promoter, and used the resulting vectors to infect fertilized murine oocytes. LacZ staining of E11 embryos obtained by first using the vectors in pools and then testing individual candidates led to the identification of 3 enhancers, only one of which shows significant evolutionary conservation. In situ hybridization and 3C/4C experiments suggest that this enhancer, which is active in the neural tube and posterior diencephalon, influences the expression of the Olig1 and/or Olig2 genes. This work provides a new approach for the large-scale in vivo screening of transcriptional regulatory sequences, and further demonstrates that evolutionary conservation alone seems too limiting a criterion for the identification of enhancers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.