ArticleGenes & development2011
Initial transcribed region sequences influence the composition and functional properties of the bacterial elongation complex.
Article in Genes & development, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Article
- Promoter-proximal transcription-translation coupling controls early transcription in Escherichia coli.Nucleic acids research · 2025Article
- Automated model-predictive design of synthetic promoters to control transcriptional profiles in bacteria.Nature communications · 2022Article
- Structural basis of transcriptional regulation by a nascent RNA element, HK022 putRNA.Nature communications · 2022Article
- Structural advances in transcription elongation.Current opinion in structural biology · 2022Review
- Structural and mechanistic basis of σ-dependent transcriptional pausing.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Nascent RNA sequencing identifies a widespread sigma70-dependent pausing regulated by Gre factors in bacteria.Nature communications · 2021Article
- The Role of Pyrophosphorolysis in the Initiation-to-Elongation Transition by E. coli RNA Polymerase.Journal of molecular biology · 2019Article
- RNA Polymerase Clamp Movement Aids Dissociation from DNA but Is Not Required for RNA Release at Intrinsic Terminators.Journal of molecular biology · 2019Article
- Delayed inhibition mechanism for secondary channel factor regulation of ribosomal RNA transcription.eLife · 2019Article
- DNA template sequence control of bacterial RNA polymerase escape from the promoter.Nucleic acids research · 2018Article
- Effects of mRNA Degradation and Site-Specific Transcriptional Pausing on Protein Expression Noise.Biophysical journal · 2018Article
- Review
- σ38-dependent promoter-proximal pausing by bacterial RNA polymerase.Nucleic acids research · 2017Article
- A universal transcription pause sequence is an element of initiation factor σ70-dependent pausing.Nucleic acids research · 2016Article
- Nascent RNA length dictates opposing effects of NusA on antitermination.Nucleic acids research · 2016Article
- Bacterial RNA polymerase can retain σ70 throughout transcription.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Article
- Two transcription pause elements underlie a σ70-dependent pause cycle.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Distinct functions of the RNA polymerase σ subunit region 3.2 in RNA priming and promoter escape.Nucleic acids research · 2014Article
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Authors and funding
4 authors.
Funding
Abstract
The bacterial RNA polymerase (RNAP) holoenzyme consists of a catalytic core enzyme (α(2)ββ'ω) in complex with a σ factor that is essential for promoter recognition and transcription initiation. During early elongation, the stability of interactions between σ and the remainder of the transcription complex decreases. Nevertheless, there is no mechanistic requirement for release of σ upon the transition to elongation. Furthermore, σ can remain associated with RNAP during transcription elongation and influence regulatory events that occur during transcription elongation. Here we demonstrate that promoter-like DNA sequence elements within the initial transcribed region that are known to induce early elongation pausing through sequence-specific interactions with σ also function to increase the σ content of downstream elongation complexes. Our findings establish σ-dependent pausing as a mechanism by which initial transcribed region sequences can influence the composition and functional properties of the transcription elongation complex over distances of at least 700 base pairs.
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